Cui Hao, Zhang Zhibin, Xu Xin, Zuo Kejing, Ji Jun, Guo Ge, Kan Yunchao, Yao Lunguang, Xie Qingmei, Bi Yingzuo
Henan Provincial Engineering Laboratory of Insects Bio-reactor, Nanyang Normal University, Nanyang, China.
Laboratory Animal Center, Chifeng Municipal Hospital, Chifeng, China.
Front Vet Sci. 2023 Oct 3;10:1252628. doi: 10.3389/fvets.2023.1252628. eCollection 2023.
(feline chaphamaparvovirus, FeChPV) is a novel feline parvovirus originally detected in Canadian cats in 2019, and it has also been identified in domestic cats in other nations. To evaluate the prevalence and genetic diversity of FeChPV in China, rectal swabs of pet cats from Henan, Guangdong, Anhui, Zhejiang, and Inner Mongolia provinces were collected. Of the 230 samples subjected to nested polymerase chain reaction, 6 (2.6%) tested positive for FeChPV. Although all positive samples were from cats with diarrhea, statistical analyses revealed no correlation between the presence of the virus and clinical symptoms ( > 0.05). Phylogenetic trees of nonstructural protein 1 (NS1) and capsid protein (VP1) demonstrated that these six new strains formed a major branch with other reference FeChPV strains and considerably differed from 1. Moreover, recombination analysis revealed that the FeChPV strain CHN20201025, previously detected in a dog, was a recombinant and strains CHN200228 and CHN180917, identified in this study, were the closest relatives to the parental strains. The findings of this study and a previous study wherein FeChPV was detected in dogs suggest that FeChPV can propagate between species. Additionally, these findings indicate that the genetic diversity of FeChPV can provide an insight into the epidemiological status of FeChPV in China.
猫查帕马细小病毒（FeChPV）是一种新型猫细小病毒，于2019年在加拿大猫中首次检测到，其他国家的家猫中也有发现。为评估FeChPV在中国的流行情况和遗传多样性，收集了河南、广东、安徽、浙江和内蒙古等省份宠物猫的直肠拭子。在230份进行巢式聚合酶链反应的样本中，6份（2.6%）检测出FeChPV呈阳性。尽管所有阳性样本均来自腹泻猫，但统计分析显示病毒的存在与临床症状之间无相关性（P>0.05）。非结构蛋白1（NS1）和衣壳蛋白（VP1）的系统发育树表明，这六个新毒株与其他参考FeChPV毒株形成一个主要分支，与毒株1有很大差异。此外，重组分析显示，先前在一只狗中检测到的FeChPV毒株CHN20201025是一个重组体，本研究中鉴定的毒株CHN200228和CHN180917是亲本毒株的近亲。本研究以及先前在狗中检测到FeChPV的研究结果表明，FeChPV可以在物种间传播。此外，这些发现表明FeChPV的遗传多样性可以为了解其在中国的流行病学状况提供线索。
