Liang Dong, Zhang Xiaoyi, Wang Yi, Huo Taotao, Qian Min, Xie Yibo, Li Wenshuai, Yu Yunqiu, Shi Wei, Liu Qianqian, Zhu Junle, Luo Chun, Cao Zhijuan, Huang Rongqin
School of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai, 201203, China.
Center for Advanced Low-dimension Materials, State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201600, China.
Bioact Mater. 2021 Dec 18;14:145-151. doi: 10.1016/j.bioactmat.2021.11.033. eCollection 2022 Aug.
Sensitive detection and accurate diagnosis/prognosis of glioma remain urgent challenges. Herein, dispersed magnetic covalent organic framework nanospheres (MCOF) with uniformed FeO nano-assembly as cores and high-crystalline COF as shells were prepared by monomer-mediated in-situ interface growth strategy. Based on the unique interaction between MCOF and hairpin DNA, a fluorescent signal amplified miRNA biosensor was constructed. It could realize the sensitive detection of miRNA-182 in different matrixes, where the detection limit, linearity range and determination coefficient (R) in real blood samples reached 20 fM, 0.1 pM-10 pM and 0.991, respectively. Also, it possessed good stability and precision as observed from the low intra-day/inter-day RSD and high extraction recovery. As a result, it could quantify miRNA-182 in serum of glioma patients, the concentration of which was significantly higher than that of healthy people and obviously decreased after surgery. Finally, a proof-of-concept capillary chip system using this biosensor was proposed to realize the visualized detection of miRNA-182 in microsample. These findings suggest a robust way for sensitive detection and accurate diagnosis/prognosis of glioma.
胶质瘤的灵敏检测及准确诊断/预后评估仍然是亟待解决的挑战。在此，通过单体介导的原位界面生长策略制备了以均匀的FeO纳米聚集体为核、高结晶度的共价有机框架（COF）为壳的分散磁性共价有机框架纳米球（MCOF）。基于MCOF与发夹DNA之间的独特相互作用，构建了一种荧光信号放大的miRNA生物传感器。它能够实现对不同基质中miRNA-182的灵敏检测，其中实际血液样本中的检测限、线性范围和决定系数（R）分别达到20 fM、0.1 pM - 10 pM和0.991。此外，从低日内/日间相对标准偏差（RSD）和高萃取回收率可以看出，它具有良好的稳定性和精密度。结果，它能够对胶质瘤患者血清中的miRNA-182进行定量，其浓度显著高于健康人，且术后明显降低。最后，提出了一种使用该生物传感器的概念验证毛细管芯片系统，以实现对微量样本中miRNA-182的可视化检测。这些发现为胶质瘤的灵敏检测及准确诊断/预后评估提供了一种有力的方法。
