Laboratory of Genetics, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
Université Paris Cité, INSERM U1266, Institute of Psychiatry and Neuroscience of Paris, Laboratory of Dynamics of Neuronal Structure in Health and Disease, 102 rue de la Santé, 75014 Paris, France.
STAR Protoc. 2022 Mar 17;3(2):101261. doi: 10.1016/j.xpro.2022.101261. eCollection 2022 Jun 17.
Astrocytes are an essential component of the central nervous system for neuronal support and response to injury and disease. Here, we present a protocol to generate glial progenitor cells (GPCs) from human-induced pluripotent stem cells (iPSCs) that can be further differentiated into inflammation-responsive astrocytes. This two-step protocol has the advantage of reducing the time of astrocyte differentiation since GPCs can be frozen and stored. For complete details on the use and execution of this protocol, please refer to Santos et al. (2017) and Vadodaria et al. (2021).
星形胶质细胞是中枢神经系统的重要组成部分，具有支持神经元和对损伤及疾病作出反应的功能。在此，我们提供了一种从人诱导多能干细胞（iPSC）生成神经胶质前体细胞（GPC）的方案，这些细胞可以进一步分化为对炎症有反应的星形胶质细胞。该两步法的优点是减少了星形胶质细胞分化的时间，因为 GPC 可以冷冻和储存。如需了解本方案的使用和实施的详细信息，请参考 Santos 等人（2017 年）和 Vadodaria 等人（2021 年）的研究。
