Tao Xingkui, Zhang Xingtao, Feng Fan
School of Biological and Food Engineering, Suzhou University.
Biol Pharm Bull. 2022 May 1;45(5):553-560. doi: 10.1248/bpb.b21-00634. Epub 2022 Mar 18.
Non-small cell lung cancer (NSCLC) accounts for approximately 85% of lung cancer-related mortality, and it has a high risk of early recurrence and distant metastasis. The prerequisite for the deterioration of NSCLC is the malignant proliferation and migration of cancer cells, and in this study Astragalus membranaceus polysaccharides (APS) was firstly showed that it could decrease the cell proliferation of A549 and NCI-H1299. Through bioinformatics analysis, the up-regulation of miR-195-5p was positively correlated with the survival rate of lung cancer patients. Real-time PCR indicated APS could increase the expression level of miR-195-5p, and the miR-195-5p inhibitor was used to verify that it could reverse the inhibitory effect of Astragalus polysaccharide on lung cancer cell migration and invasion. Therefore, we believe that APS could inhibit the proliferation and migration of NSCLC cells by regulating miR-195-5p, which laid the foundation for further research on the functional mechanism of miR-195-5p in NSCLC.
非小细胞肺癌（NSCLC）约占肺癌相关死亡率的85%，且具有早期复发和远处转移的高风险。NSCLC恶化的前提是癌细胞的恶性增殖和迁移，在本研究中首次表明黄芪多糖（APS）可降低A549和NCI-H1299细胞的增殖。通过生物信息学分析，miR-195-5p的上调与肺癌患者的生存率呈正相关。实时PCR表明APS可增加miR-195-5p的表达水平，并使用miR-195-5p抑制剂来验证其可逆转黄芪多糖对肺癌细胞迁移和侵袭的抑制作用。因此，我们认为APS可通过调节miR-195-5p来抑制NSCLC细胞的增殖和迁移，这为进一步研究miR-195-5p在NSCLC中的功能机制奠定了基础。
