Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON, N1G 2W1, Canada.
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, N1G 2W1, Canada.
BMC Res Notes. 2022 Mar 22;15(1):111. doi: 10.1186/s13104-022-06003-5.
Canine lymphoma, the most common hematological cancer in dogs, shares many molecular and clinical characteristics with human Non-Hodgkin lymphoma (NHL). The standard treatment for canine lymphoma is "CHOP" multiagent chemotherapy protocol consisting of Cyclophosphamide, Doxorubicin (Hydroxydaunorubicin), Vincristine (Oncovin™), and Prednisone. Approximately 70-85% of patients treated with CHOP achieve clinical remission. However, duration of remission varies and the majority of dogs eventually relapse. To identify possible biomarkers for patients failing to achieve remission, we performed RNA-Seq analysis on 25 cases of canine lymphoma obtained prior the start of their CHOP therapy regime and assessed gene expression associated with patient progression free survival (PFS).
The data consists of (1) raw RNA-Seq reads in 75 bp fastq format from fine needle aspirate samples of enlarged lymph nodes from canine patients with naturally occurring lymphoma; (2) Fragments Per Kilobase Million (FPKM) values for each sample; (3) raw transcript counts for each sample; (4) anonymized patient details including PFS; (5) heat map of gene expression and (6) Cox proportional hazard analysis showing significantly expressed genes. These data may be useful for comparative analysis of gene expression in human NHL and analysis of gene expression associated with disease outcome in canine lymphoma.
犬淋巴瘤是犬最常见的血液系统癌症，与人类非霍奇金淋巴瘤（NHL）具有许多分子和临床特征。犬淋巴瘤的标准治疗方法是“CHOP”多药化疗方案，包括环磷酰胺、多柔比星（羟基柔红霉素）、长春新碱（Oncovin™）和泼尼松。约 70-85%接受 CHOP 治疗的患者达到临床缓解。然而，缓解持续时间不同，大多数狗最终会复发。为了确定无法缓解的患者的可能生物标志物，我们对 25 例接受 CHOP 治疗前的犬淋巴瘤病例进行了 RNA-Seq 分析，并评估了与患者无进展生存期（PFS）相关的基因表达。
该数据包括 (1) 来自犬自然发生淋巴瘤的肿大淋巴结细针抽吸样本的 75 bp fastq 格式原始 RNA-Seq reads；(2) 每个样本的每千碱基百万片段数 (FPKM) 值；(3) 每个样本的原始转录计数；(4) 包括 PFS 在内的匿名患者详细信息；(5) 基因表达热图和 (6) 显示显著表达基因的 Cox 比例风险分析。这些数据可能有助于对人类 NHL 中的基因表达进行比较分析，并分析犬淋巴瘤中与疾病结果相关的基因表达。
