Department of Biotechnology, Institute of Graduate Studies and Research (IGSR), Alexandria University, Alexandria, Egypt.
Department of Medical Biotechnology, Institute of Genetic Engineering, City of Scientific Research and Technological Applications, Alexandria, Egypt.
Sci Rep. 2022 Mar 22;12(1):4871. doi: 10.1038/s41598-022-08880-z.
Nile Tilapia fish scale collagen has high biodegradability, excellent biocompatibility, and low antigenicity. We assessed both the encapsulation efficiency of theophylline into Nile Tilapia fish scale-based collagen nanoparticles and their stability as a pulmonary drug delivery system in male Sprague-Dawley rats. The present study has demonstrated the successful encapsulation of theophylline into the synthesised nanoparticles as shown by spectrophotometric analysis, light microscope, scanning electron microscope, transmission electron microscope, and dynamic light scattering. The antibacterial activity of the nanoparticles improves with increasing their concentrations. Intratracheal treatment of rats using theophylline-encapsulated nanoparticles reduced the levels of creatinine, alanine transaminase, and aspartate transaminase, compared to the control group. Nevertheless, nanoparticles combined with theophylline exhibited no effects on cholesterol and triglycerides levels. Histopathological examination revealed typical uniform and diffuse thickening of the alveolar walls with capillary oedema in treated rats. We concluded that the synthesised collagen nanoparticles appropriately target the lungs of male Sprague-Dawley rats when delivered via a nebuliser, showing good tolerability to lung cells. However, dose ratio of collagen nanoparticles to theophylline needs further evaluation. The nanoprecipitation method may be optimised to involve poorly water-soluble inhaled drugs, and avoid the drawbacks of traditional drug delivery.
罗非鱼鱼鳞胶原蛋白具有良好的生物降解性、优异的生物相容性和低抗原性。我们评估了茶碱被包裹在罗非鱼鱼鳞胶原蛋白纳米颗粒中的包封效率及其作为肺部给药系统在雄性 Sprague-Dawley 大鼠中的稳定性。本研究通过分光光度分析、光学显微镜、扫描电子显微镜、透射电子显微镜和动态光散射成功地将茶碱包裹在合成的纳米颗粒中。纳米颗粒的抗菌活性随着浓度的增加而提高。与对照组相比，用茶碱包封的纳米颗粒经气管内治疗大鼠，可降低肌酸酐、丙氨酸转氨酶和天冬氨酸转氨酶的水平。然而，纳米颗粒与茶碱结合对胆固醇和甘油三酯水平没有影响。组织病理学检查显示，治疗组大鼠的肺泡壁出现典型的均匀弥漫性增厚，并伴有毛细血管水肿。我们得出结论，当通过雾化器输送时，合成的胶原蛋白纳米颗粒可以将药物靶向雄性 Sprague-Dawley 大鼠的肺部，对肺细胞具有良好的耐受性。然而，还需要进一步评估胶原蛋白纳米颗粒与茶碱的剂量比。纳米沉淀法可用于优化包含疏水性吸入性药物，并避免传统药物输送的缺点。
