Institut National de la Santé et de la Recherche Médicale (INSERM), U1215 NeuroCentre Magendie, France; University of Bordeaux, Bordeaux 33077, France.
Institut National de la Santé et de la Recherche Médicale (INSERM), U1215 NeuroCentre Magendie, France; University of Bordeaux, Bordeaux 33077, France.
J Neurosci Methods. 2022 May 1;373:109560. doi: 10.1016/j.jneumeth.2022.109560. Epub 2022 Mar 20.
Mitochondrial calcium handling is a particularly active research area in the neuroscience field, as it plays key roles in the regulation of several functions of the central nervous system, such as synaptic transmission and plasticity, astrocyte calcium signaling, neuronal activity… In the last few decades, a panel of techniques have been developed to measure mitochondrial calcium dynamics, relying mostly on photonic microscopy, and including synthetic sensors, hybrid sensors and genetically encoded calcium sensors. The goal of this review is to endow the reader with a deep knowledge of the historical and latest tools to monitor mitochondrial calcium events in the brain, as well as a comprehensive overview of the current state of the art in brain mitochondrial calcium signaling. We will discuss the main calcium probes used in the field, their mitochondrial targeting strategies, their key properties and major drawbacks. In addition, we will detail the main roles of mitochondrial calcium handling in neuronal tissues through an extended report of the recent studies using mitochondrial targeted calcium sensors in neuronal and astroglial cells, in vitro and in vivo.
线粒体钙处理是神经科学领域一个特别活跃的研究领域，因为它在调节中枢神经系统的几个功能中起着关键作用，如突触传递和可塑性、星形胶质细胞钙信号、神经元活动等。在过去几十年中，已经开发出了一系列测量线粒体钙动力学的技术，主要依赖于光子显微镜，包括合成传感器、杂交传感器和基因编码钙传感器。本综述的目的是使读者深入了解监测大脑中线粒体钙事件的历史和最新工具，以及大脑中线粒体钙信号的最新技术现状。我们将讨论该领域中使用的主要钙探针、它们的线粒体靶向策略、它们的关键特性和主要缺点。此外，我们将通过详细报告最近使用线粒体靶向钙传感器在神经元和星形胶质细胞中的研究，深入探讨线粒体钙处理在神经元组织中的主要作用，无论是在体外还是在体内。
