Bracesco Nelson, Dell Mercedes, Rocha Alberto, Behtash Solmaz, Menini Teresita, Gugliucci Alejandro, Nunes Elia
Laboratory de Radiobiología, Department of Biofísica, Facultad Medicina, University of the República, Montevideo, Uruguay.
J Altern Complement Med. 2003 Jun;9(3):379-87. doi: 10.1089/107555303765551606.
We analyzed the antioxidant properties of Ilex paraguariensis infusion (Ip) popularly known as mate (mä'tā), by using two experimental models: the induction of DNA double-strand breaks (DSB) by hydrogen peroxide (H(2)O(2)) and lethality in Saccharomyces cerevisiae, as well as peroxide and lipoxygenase-induced human low-density lipoprotein (LDL) oxidation. Diploid yeast cells were exposed to different concentrations of H(2)O(2) (5-10 mmol/L) in the absence or presence of Ip infusion (10(-1) v/v) or alpha-tocopherol (10(-2) mol/L). Both mate infusion and alpha-tocopherol significantly decreased the dose dependent DSB number, and the lethality induced by H(2)O(2). Peroxynitrite and lipoxygenase-induced human LDL oxidation are inhibited by Ip extracts in a potent, dose-dependent fashion. Dilutions of 5 x 10(-3) v/v provide 50% +/- 10% inhibition. Finally, Ip extracts are potent direct quenchers of the free radical 1,1-diphenyl-2-picrylhydrazyl. Dilutions of 2 x 10(-2) v/v produced quenching of more than 30%, which was comparable to that obtained with 0.5-1 mmol/L alpha-tocopherol or the quercetin aglycone, respectively. For comparison, total polyphenol content of Ip, green, and black tea (Camelia sinensis) were 6.5 +/- 0.8; 1.8 +/- 0.5; and 1.13 +/- 0.3 mmol of quercetin equivalents per liter, respectively. Their respective free radical quenching activities at dilutions of 1 x 10(-1) v/v were 75% +/- 5%; 35% +/- 5%; and 2% +/- 5%. Ip is thus a rich source of polyphenols and has antioxidant properties comparable to those of green tea which merit further in vivo intervention and cross-sectional studies.
我们通过两种实验模型分析了巴拉圭冬青浸液（Ip）（俗称马黛茶）的抗氧化特性：过氧化氢（H₂O₂）诱导的DNA双链断裂（DSB）以及酿酒酵母中的致死率，还有过氧化物和脂氧合酶诱导的人低密度脂蛋白（LDL）氧化。将二倍体酵母细胞暴露于不同浓度的H₂O₂（5 - 10 mmol/L）中，同时存在或不存在Ip浸液（10⁻¹ v/v）或α-生育酚（10⁻² mol/L）。马黛茶浸液和α-生育酚均显著降低了剂量依赖性的DSB数量以及H₂O₂诱导的致死率。过氧亚硝酸盐和脂氧合酶诱导的人LDL氧化受到Ip提取物的有效抑制，且呈剂量依赖性。5×10⁻³ v/v的稀释液可提供50%±10%的抑制率。最后，Ip提取物是自由基1,1-二苯基-2-苦基肼的有效直接淬灭剂。2×10⁻² v/v的稀释液产生了超过30%的淬灭率，分别与0.5 - 1 mmol/L的α-生育酚或槲皮素苷元相当。作为比较，Ip、绿茶和红茶（茶树）的总多酚含量分别为每升6.5±0.8、1.8±0.5和1.13±0.3 mmol槲皮素当量。它们在1×10⁻¹ v/v稀释液下各自的自由基淬灭活性分别为75%±5%、35%±5%和2%±5%。因此，Ip是多酚的丰富来源，具有与绿茶相当的抗氧化特性，值得进一步进行体内干预和横断面研究。
