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膳食补充功能性成分对猪背最长肌脂类变化的分子研究

A molecular insight into the lipid changes of pig Longissimus thoracis muscle following dietary supplementation with functional ingredients.

机构信息

Department of Animal Science, Food and Nutrition, Università Cattolica del Sacro Cuore, Piacenza, Italy.

Interdepartmental centre for Industrial Agrifood research (CIRI-AGRO)-Università di Bologna, Cesena, Italy.

出版信息

PLoS One. 2022 Mar 24;17(3):e0264953. doi: 10.1371/journal.pone.0264953. eCollection 2022.

DOI:10.1371/journal.pone.0264953
PMID:35324931
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8947141/
Abstract

In this work, the Longissimus thoracis pig skeletal muscle was used as a model to investigate the impact of two different diets, supplemented with n-3 polyunsaturated fatty acids from extruded linseed (L) and polyphenols from grape skin and oregano extracts (L+P), on the lipidomic profile of meat. A standard diet for growing-finishing pigs (CTRL) was used as a control. Changes in lipids profile were investigated through an untargeted lipidomics and transcriptomics combined investigation. The lipidomics identified 1507 compounds, with 195 compounds fitting with the MS/MS spectra of LipidBlast database. When compared with the CTRL group, the L+P diet significantly increased 15 glycerophospholipids and 8 sphingolipids, while the L diet determined a marked up-accumulation of glycerolipids. According to the correlations outlined between discriminant lipids and genes, the L diet may act preventing adipogenesis and the related inflammation processes, while the L+P diet promoted the expression of genes involved in lipids' biosynthesis and adipogenic extracellular matrix formation and functioning.

摘要

本研究以猪背最长肌为模型，研究了两种不同饮食（补充挤压亚麻籽中的 n-3 多不饱和脂肪酸（L）和葡萄皮及牛至提取物中的多酚（L+P））对肉脂代谢组学特征的影响。以生长育肥猪的标准日粮（CTRL）作为对照。通过非靶向脂质组学和转录组学联合研究来探究脂质谱的变化。脂质组学共鉴定到 1507 种化合物，其中 195 种化合物与 LipidBlast 数据库的 MS/MS 谱相匹配。与 CTRL 组相比，L+P 日粮显著增加了 15 种甘油磷脂和 8 种鞘脂，而 L 日粮则显著增加了甘油酯的积累。根据区分性脂质和基因之间的关系，L 日粮可能具有预防脂肪生成和相关炎症过程的作用，而 L+P 日粮则促进了参与脂质生物合成以及脂肪生成细胞外基质形成和功能的基因的表达。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a99/8947141/fc5674375a9b/pone.0264953.g008.jpg
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