Yu Tzu-Jung, Tang Jen-Yang, Shiau Jun-Ping, Hou Ming-Feng, Yen Chia-Hung, Ou-Yang Fu, Chen Chung-Yi, Chang Hsueh-Wei
Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
School of Post-Baccalaureate Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
Antioxidants (Basel). 2022 Mar 19;11(3):587. doi: 10.3390/antiox11030587.
Ginger is a popular spice and consists of several bioactive antioxidant compounds. Gingerenone A (Gin A), a novel compound isolated from , is rarely investigated for its anti-breast-cancer properties. Some ginger extracts have been reported to initiate senescence, an anticancer strategy. However, the anticancer effects of Gin A on breast cancer cells remain unclear. The present study aims to assess the modulating impact of Gin A acting on proliferation and senescence to breast cancer cells. Gin A diminished the cellular ATP content and decreased the cell viability of the MTS assay in several breast cancer cell lines. It also showed a delayed G2/M response to breast cancer cells (MCF7 and MDA-MB-231). -acetylcysteine (NAC), an oxidative stress inhibitor, can revert these responses of antiproliferation and G2/M delay. The oxidative stress and senescence responses of Gin A were further validated by increasing reactive oxygen species, mitochondrial superoxide, and β-galactosidase activity, which were reverted by NAC. Gin A also upregulated senescence-associated gene expressions. In addition to oxidative stress, Gin A also induced DNA damage responses by increasing γH2AX level and foci and generating 8-hydroxyl-2'-deoxyguanosine in breast cancer cells, which were reverted by NAC. Therefore, Gin A promotes antiproliferation and senescence of breast cancer cells induced by oxidative stress.
生姜是一种广受欢迎的香料，含有多种具有生物活性的抗氧化化合物。姜烯酮A（Gin A）是一种从[具体来源未给出]中分离出的新型化合物，其抗乳腺癌特性鲜少被研究。一些生姜提取物据报道可引发细胞衰老，这是一种抗癌策略。然而，Gin A对乳腺癌细胞的抗癌作用仍不清楚。本研究旨在评估Gin A对乳腺癌细胞增殖和衰老的调节作用。Gin A降低了几种乳腺癌细胞系中的细胞ATP含量，并降低了MTS法检测的细胞活力。它还显示出对乳腺癌细胞（MCF7和MDA - MB - 231）的G2/M期反应延迟。抗氧化应激抑制剂N - 乙酰半胱氨酸（NAC）可逆转这些抗增殖和G2/M期延迟反应。通过增加活性氧、线粒体超氧化物和β - 半乳糖苷酶活性进一步验证了Gin A的氧化应激和衰老反应，而这些反应可被NAC逆转。Gin A还上调了衰老相关基因的表达。除氧化应激外，Gin A还通过增加γH2AX水平和病灶以及在乳腺癌细胞中产生8 - 羟基 - 2'- 脱氧鸟苷来诱导DNA损伤反应，这些反应可被NAC逆转。因此，Gin A促进由氧化应激诱导的乳腺癌细胞的抗增殖和衰老。
