Warman Dwina Juliana, Jia Huijuan, Kato Hisanori
Health Nutrition, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Department of Applied Nutrition, School of Nutrition, Kagawa Nutrition University, 3-9-21 Chiyoda, Sakado-shi 350-0288, Japan.
Antioxidants (Basel). 2023 May 30;12(6):1178. doi: 10.3390/antiox12061178.
Chronological aging is commonly accompanied by chronic low-grade inflammation (or "inflammaging"), a contributor to the development of age-related chronic diseases. Aging increases oxidative stress that accelerates telomere shortening, leading to cell senescence and the generation of senescence-associated secretory phenotype (SASP) that exacerbates inflammation. Dietary antioxidants may help protect telomeres and attenuate inflammation. Thyme essential oil (TEO), reported for its potency against neuroinflammation, was fed to chronologically aged C57BL/6J mice for 24 weeks. The TEO diet showed notable impacts on the hippocampus, indicated by lower expression of the aging-related gene ( = 0.0783) and significantly lower expression of cyclin D kinase and ( < 0.05) compared to the age-matched control mice. The TEO group also showed significantly lower gene expression of the pro-inflammatory cytokine ( < 0.05) in the hippocampus and lower expression in the liver and cerebellum ( < 0.05). In vitro experiments conducted on NIH-3T3 cells expressing SASP revealed the dose-dependent anti-inflammatory activity of TEO. Remarkably, TEO diet-fed mice showed higher survival rates and significantly longer blood telomere lengths than the control mice. Monoterpene antioxidants in TEO, particularly thymol and p-cymene, may primarily contribute to the anti-inflammatory and telomere-protecting activities of TEO.
自然衰老通常伴随着慢性低度炎症（即“炎症衰老”），这是导致与年龄相关的慢性疾病发展的一个因素。衰老会增加氧化应激，加速端粒缩短，导致细胞衰老以及衰老相关分泌表型（SASP）的产生，从而加剧炎症。膳食抗氧化剂可能有助于保护端粒并减轻炎症。据报道百里香精油（TEO）具有抗神经炎症的功效，将其喂食给自然衰老的C57BL/6J小鼠24周。与年龄匹配的对照小鼠相比，TEO饮食对海马体产生了显著影响，表现为衰老相关基因的表达降低（ = 0.0783），细胞周期蛋白D激酶和的表达显著降低（ < 0.05）。TEO组在海马体中促炎细胞因子的基因表达也显著降低（ < 0.05），在肝脏和小脑中的表达也降低（ < 0.05）。对表达SASP的NIH-3T3细胞进行的体外实验揭示了TEO的剂量依赖性抗炎活性。值得注意的是，喂食TEO饮食的小鼠比对照小鼠表现出更高的存活率和显著更长的血液端粒长度。TEO中的单萜类抗氧化剂，特别是百里香酚和对伞花烃，可能是TEO抗炎和保护端粒活性的主要贡献者。
