Alves Fernanda, Gomes Guimarães Gabriela, Mayumi Inada Natália, Pratavieira Sebastião, Salvador Bagnato Vanderlei, Kurachi Cristina
Optics Group from São Carlos Institute of Physics (IFSC), University of São Paulo (USP), Av. Trabalhador São-carlense, 400-Centro, CEP 13560-970, São Carlos, SP, Brazil.
The Department of Biomedical Engineering, College of Engineering, Texas A&M University, 101 Bizzell Street, College Station, Texas, 77843.
Lasers Surg Med. 2021 Oct;53(8):1113-1121. doi: 10.1002/lsm.23383. Epub 2021 Jan 28.
This work evaluated antimicrobial photodynamic therapy (PDT), sonodynamic therapy (SDT), and the association of both therapies (sonophotodynamic therapy [SPDT]), mediated by curcumin (Cur) against Staphylococcus aureus biofilm. Next, additional strategies for these treatments were assessed.
S. aureus biofilms received PDT, SDT, and SPDT, mediated by Cur (80 µM), LED light (450 nm), and 1 MHz ultrasound. The same treatments were also performed adding a strategy: Cur with sodium dodecyl sulfate (SDS), Cur with potassium iodide (KI) or a pre-treatment with ultrasound. Cell viability was determined and biofilm architecture was evaluated under confocal microscopy.
SPDT was more effective to inactivate the bacteria than PDT and SDT. SDS achieved the greatest viability reductions, followed by KI and ultrasound pre-treatment. Confocal images revealed biofilm disruption and a reduced number of cells in all treatments. However, SPDT exhibited a pronounced effect and it was greater using SDS.
SPDT was more effective and additional strategies potentiated its effectiveness. Lasers Surg. Med. © 2021 Wiley Periodicals LLC.
本研究评估了由姜黄素（Cur）介导的抗菌光动力疗法（PDT）、声动力疗法（SDT）以及这两种疗法的联合（声光动力疗法[SPDT]）对金黄色葡萄球菌生物膜的作用。接下来，评估了这些治疗方法的其他策略。
金黄色葡萄球菌生物膜接受由Cur（80µM）、发光二极管（LED）光（450nm）和1MHz超声介导的PDT、SDT和SPDT。同样的治疗也采用了一种策略：Cur与十二烷基硫酸钠（SDS）联合、Cur与碘化钾（KI）联合或超声预处理。测定细胞活力，并在共聚焦显微镜下评估生物膜结构。
SPDT比PDT和SDT更有效地使细菌失活。SDS导致的活力降低最大，其次是KI和超声预处理。共聚焦图像显示所有治疗中生物膜均被破坏且细胞数量减少。然而，SPDT表现出显著效果，使用SDS时效果更明显。
SPDT更有效，其他策略增强了其有效性。《激光外科与医学》。©2021威利期刊有限责任公司。
