Egan J E, Haynes J D, Brown N D, Eisemann C S
Am J Trop Med Hyg. 1986 Sep;35(5):890-7. doi: 10.4269/ajtmh.1986.35.890.
Human retroplacental serum (RPS) containing polyamine oxidase inhibited the growth of the Camp strain of Plasmodium falciparum in vitro as assayed by the parasite's decreased incorporation of 3H-hypoxanthine. Inhibition was dose-dependent on the concentrations of serum polyamine oxidase and added polyamines. Almost complete inhibition was seen in 96-hr asynchronous cultures containing 10% RPS and in those containing 1.2% RPS plus 50 microM polyamine. Subtle morphologic changes in mature stages and decreased numbers of new rings were associated with inhibition seen in 19-hr synchronous cultures initiated at the trophozoite stage. These incubation times were longer than in previous reports showing inhibition of malaria parasites by bovine polyamine oxidase but not by human polyamine oxidase. Macrophages contain polyamine oxidase, the reaction products of which are known to be similar to those of RPS polyamine oxidase but different from those of bovine polyamine oxidase. It remains to be determined whether human polyamine oxidase, acting upon ubiquitous polyamines, contributes to host defenses against malaria.
含多胺氧化酶的人胎盘后血清（RPS）在体外抑制了恶性疟原虫坎普株的生长，这是通过寄生虫对3H-次黄嘌呤摄取减少来测定的。抑制作用呈剂量依赖性，取决于血清多胺氧化酶和添加的多胺浓度。在含有10%RPS的96小时非同步培养物以及含有1.2%RPS加50微摩尔多胺的培养物中观察到几乎完全抑制。在滋养体阶段开始的19小时同步培养物中，成熟阶段的细微形态变化和新环数量的减少与抑制作用相关。这些孵育时间比以前报道的牛多胺氧化酶抑制疟原虫但人多胺氧化酶不抑制疟原虫的时间更长。巨噬细胞含有多胺氧化酶，其反应产物已知与RPS多胺氧化酶的反应产物相似，但与牛多胺氧化酶的不同。作用于普遍存在的多胺的人多胺氧化酶是否有助于宿主抵抗疟疾还有待确定。
