基因组的比较分析揭示了生物技术应用的遗传潜力。

A Comparative Analysis of Genomes Unravels the Genetic Potential for Biotechnological Applications.

机构信息

Dipartimento di Biologia, University of Naples Federico II, 80126 Naples, Italy.

Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

出版信息

Int J Mol Sci. 2022 Mar 15;23(6):3135. doi: 10.3390/ijms23063135.

DOI:10.3390/ijms23063135

PMID:35328559

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8954581/

Abstract

The production of biochemicals requires the use of microbial strains with efficient substrate conversion and excellent environmental robustness, such as species. So far, the genomes of 47 strains have been sequenced. Herein, we report a comparative genomic analysis of nine strains on the full repertoire of Carbohydrate-Active enZymes (CAZymes), secretion systems, and resistance mechanisms to environmental challenges. Moreover, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) immune system along with CRISPR-associated (Cas) genes, was also analyzed. Overall, this study expands our understanding of the strain's genomic diversity of to fully exploit its potential in biotechnological applications.

摘要

生物化学制品的生产需要利用具有高效底物转化和出色环境稳健性的微生物菌株，如物种。到目前为止，已经对 47 株菌株的基因组进行了测序。在此，我们报告了对 9 株菌株的全谱碳水化合物活性酶（CAZymes）、分泌系统和对环境挑战的抗性机制的比较基因组分析。此外，还分析了规律成簇间隔短回文重复（CRISPR）免疫系统以及与之相关的（Cas）基因。总的来说，这项研究扩展了我们对菌株基因组多样性的理解，以充分挖掘其在生物技术应用中的潜力。