定量诺如病毒载量不受粪便家庭储存的影响。
Quantitative norovirus viral load is not affected by home storage of stool.
机构信息
Divisions of Infectious Diseases, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA.
Divisions of Organ Transplantation, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA.
出版信息
Transpl Infect Dis. 2022 Jun;24(3):e13826. doi: 10.1111/tid.13826. Epub 2022 Apr 1.
Abstract
In preparation of a clinical trial of norovirus treatment, there were concerns raised by FDA about risk of self-storage of stool from patients infected with norovirus affecting quantitative assessments of norovirus RNA. Specifically, most home freezers are frost-free and may expose the samples to multiple rounds of freeze-thaw. Stool samples collected by the study team were stored at different lengths in a frost-free freezer and at -80°C. Quantitative PCRs of norovirus were performed on all samples using the same assay. By all measures, there was no significant change in measured viral load with home storage.
摘要
在准备诺如病毒治疗的临床试验时，FDA 对患者自行储存感染诺如病毒的粪便可能会影响诺如病毒 RNA 的定量评估的风险提出了担忧。具体来说，大多数家用冰箱都是无霜的，这可能会使样本多次经历冷冻和解冻。研究小组收集的粪便样本在无霜冰箱和-80°C 下储存了不同的时间长度。所有样本均使用相同的检测方法进行诺如病毒的定量 PCR。所有检测结果显示，在家中储存粪便不会对病毒载量的测量值产生显著变化。
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