Hawkins K N
Curr Eye Res. 1986 Sep;5(9):655-63. doi: 10.3109/02713688609015133.
The extent of plasma proteins in the vitreous humor of the rat was determined. Disc gel electrophoresis of the soluble proteins of the rat vitreous humor showed many protein bands, but most of the bands did not coincide with those of rat plasma. Using antibodies to rat plasma proteins, immunological analyses of vitreous and plasma, including fractions separated by high-pressure gel permeation chromatography, showed that several vitreous proteins react with plasma protein antibodies. However, only albumin and transferrin were found to be completely identical in both fluids. The concentration of albumin in vitreous was about 625 micrograms/ml and that of transferrin was 61.6 +/- 19.1 (S.D.) microgram/ml. The total protein concentration in vitreous was 9.5 +/- 0.5 (S.D.) mg/ml. The ratio of transferrin to albumin in the vitreous was approximate to that of plasma, but the sum of the two proteins accounted for less than 10% of the total protein. These data indicate that in the rat some plasma proteins, particularly albumin and transferrin, are found in the vitreous, but most of the soluble proteins appear to originate from sources other than plasma, such as the vitreous itself or in surrounding tissues including the ciliary body and the retina.
测定了大鼠玻璃体液中血浆蛋白的含量。大鼠玻璃体液可溶性蛋白的圆盘凝胶电泳显示出许多蛋白条带，但大多数条带与大鼠血浆的条带不一致。使用针对大鼠血浆蛋白的抗体，对玻璃体液和血浆进行免疫分析，包括通过高压凝胶渗透色谱分离的组分，结果显示几种玻璃体液蛋白与血浆蛋白抗体发生反应。然而，仅发现白蛋白和转铁蛋白在两种液体中完全相同。玻璃体液中白蛋白的浓度约为625微克/毫升，转铁蛋白的浓度为61.6±19.1（标准差）微克/毫升。玻璃体液中的总蛋白浓度为9.5±0.5（标准差）毫克/毫升。玻璃体液中转铁蛋白与白蛋白的比例与血浆中的比例相近，但这两种蛋白的总和占总蛋白的比例不到10%。这些数据表明，在大鼠中，玻璃体液中存在一些血浆蛋白，特别是白蛋白和转铁蛋白，但大多数可溶性蛋白似乎来源于血浆以外的其他来源，如玻璃体液本身或包括睫状体和视网膜在内的周围组织。
