益生菌菌株 10 株（OMNi BiOTiC AAD10）共培养物的组成及其发酵上清后生物效应的深入了解。

Insights into the Composition of a Co-Culture of 10 Probiotic Strains (OMNi BiOTiC AAD10) and Effects of Its Postbiotic Culture Supernatant.

机构信息

Department of Paediatric and Adolescent Surgery, Medical University of Graz, 8034 Graz, Austria.

Department of Cell Biology, Histology and Embryology, Medical University of Graz, 8034 Graz, Austria.

出版信息

Nutrients. 2022 Mar 11;14(6):1194. doi: 10.3390/nu14061194.

BACKGROUND

We aimed to gain insights in a co-culture of 10 bacteria and their postbiotic supernatant.

METHODS

Abundances and gene expression were monitored by shotgun analysis. The supernatant was characterized by liquid chromatography mass spectroscopy (LC-MS) and gas chromatography mass spectroscopy (GC-MS). Supernatant was harvested after 48 h (S48) and 196 h (S196). Susceptibility testing included nine bacteria and . Bagg albino (BALBc) mice were fed with supernatant or culture medium. Fecal samples were obtained for 16S analysis.

RESULTS

A time-dependent decrease of the relative abundances and gene expression of , , and and an increase of were observed. Substances in LC-MS were predominantly allocated to groups amino acids/peptides/metabolites and nucleotides/metabolites, relating to gene expression. Fumaric, panthotenic, 9,3-methyl-2-oxovaleric, malic and aspartic acid, cytidine monophosphate, orotidine, phosphoserine, creatine, tryptophan correlated to culture time. Supernatant had no effect against anaerobic bacteria. S48 was reactive against , , , and . S196 against and . In vivo S48/S196 had no effect on alpha/beta diversity. Linear discriminant analysis effect size (LEfSe) and analysis of composition of microbiomes (ANCOM) revealed an increase of and .

CONCLUSIONS

The postbiotic supernatant had positive antibacterial and antifungal effects in vitro and promoted the growth of distinct bacteria in vivo.

背景

我们旨在深入了解 10 种细菌及其后生元上清液的共培养物。

方法

通过 shotgun 分析监测丰度和基因表达。通过液相色谱-质谱联用（LC-MS）和气相色谱-质谱联用（GC-MS）对上清液进行表征。在 48 小时（S48）和 196 小时（S196）后收获上清液。药敏试验包括 9 种细菌和。用上清液或培养基喂养 Bagg albino（BALBc）小鼠。收集粪便样本进行 16S 分析。

结果

观察到相对丰度和基因表达的时间依赖性下降，和减少，和增加。LC-MS 中的物质主要分配到氨基酸/肽/代谢物和核苷酸/代谢物组，与基因表达相关。富马酸、泛酸、9,3-甲基-2-氧代戊酸、苹果酸和天冬氨酸、胞苷一磷酸、乳清酸、磷酸丝氨酸、肌酸、色氨酸与培养时间相关。上清液对厌氧菌没有作用。S48 对、、、有反应。S196 对、有反应。体内 S48/S196 对 alpha/beta 多样性没有影响。线性判别分析效应量（LEfSe）和微生物组组成分析（ANCOM）显示和的增加。