Institute of Physiology and Biochemistry "Ivan Đaja", Faculty of Biology, University of Belgrade, 11000 Belgrade, Serbia.
Department for Plastic and Reconstructive Surgery, Institute for Orthopedic Surgery "Banjica", 11000 Belgrade, Serbia.
Nutrients. 2022 Mar 17;14(6):1273. doi: 10.3390/nu14061273.
The present study aimed to investigate the neuroprotective effects of the vitamin B complex (B1, B2, B3, B5, B6, and B12-VBC), by studying the changes in the femoral nerve, quadriceps muscle, popliteal lymph nodes and gut microbiota in the rat model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE). VBC treatment attenuated clinical signs of EAE during the disease, and reduced the duration of EAE thereby contributing to a faster recovery. In VBC-treated EAE rats, a significant decrease in nerve and muscle nuclear density was revealed during the onset period of the disease, while a marked increase was detected at the end of the disease, compared with untreated EAE rats. In the lymph nodes of VBC-treated EAE rats, a fewer number of lymphoid follicles in the cortical area and smaller epithelioid granulomas were detected. The changes in microbiota composition were examined using 16S rRNA gene sequencing and bioinformatics analysis, which revealed the potential of VBC treatment in establishing and/or maintaining gut microbiota homeostasis. Finally, the present study demonstrated that VBC treatment ameliorated the cellular changes in the affected peripheral nerve, muscles innervated by this nerve, and the gut microbiota dysbiosis which occurred during the EAE.
本研究旨在通过研究多发性硬化症（实验性自身免疫性脑脊髓炎，EAE）大鼠模型中股神经、股四头肌、腘窝淋巴结和肠道微生物群的变化，来探讨维生素 B 复合物（B1、B2、B3、B5、B6 和 B12-VBC）的神经保护作用。VBC 治疗可减轻 EAE 期间的临床症状，并缩短 EAE 病程，从而有助于更快恢复。与未治疗的 EAE 大鼠相比，在 VBC 治疗的 EAE 大鼠中，在疾病发作期间，神经和肌肉核密度显著降低，而在疾病末期则显著增加。在 VBC 治疗的 EAE 大鼠的淋巴结中，皮质区的淋巴滤泡数量减少，上皮样肉芽肿较小。通过 16S rRNA 基因测序和生物信息学分析来检查微生物群组成的变化，结果表明 VBC 治疗具有建立和/或维持肠道微生物群平衡的潜力。最后，本研究表明，VBC 治疗可改善 EAE 期间受影响的周围神经、由该神经支配的肌肉以及肠道微生物群失调中的细胞变化。
