Plant Advanced Technologies (PAT), F-54500 Vandoeuvre-lès-Nancy, France.
Laboratoire Sols et Environnement (LSE), UAR 3562 CNRS INRAE, Université de Lorraine, F-54000 Nancy, France.
Molecules. 2022 Mar 17;27(6):1942. doi: 10.3390/molecules27061942.
Vetiver ( (L.) Roberty) is a major tropical perfume crop. Access to its essential oil (EO)-filled roots is nevertheless cumbersome and land-damaging. This study, therefore, evaluated the potential of vetiver cultivation under soilless high-pressure aeroponics (HPA) for volatile organic compound (VOC) production. The VOC accumulation in the roots was investigated by transmission electron microscopy, and the composition of these VOCs was analyzed by gas chromatography coupled with mass spectrometry (GC/MS) after sampling by headspace solid-phase microextraction (HS-SPME). The HPA-grown plants were compared to plants that had been grown in potting soil and under axenic conditions. The HPA-grown plants were stunted, demonstrating less root biomass than the plants that had been grown in potting soil. The roots were slender, thinner, more tapered, and lacked the typical vetiver fragrance. HPA cultivation massively impaired the accumulation of the less-volatile hydrocarbon and oxygenated sesquiterpenes that normally form most of the VOCs. The axenic, tissue-cultured plants followed a similar and more exacerbated trend. Ultrastructural analyses revealed that the HPA conditions altered root ontogeny, whereby the roots contained fewer EO-accumulating cells and hosted fewer and more immature intracellular EO droplets. These preliminary results allowed to conclude that HPA-cultivated vetiver suffers from altered development and root ontology disorders that prevent EO accumulation.
香根草（（L.）Roberty）是一种主要的热带香料作物。然而，获取其富含精油（EO）的根系却很麻烦，而且会破坏土地。因此，本研究评估了在无土高压气雾培（HPA）条件下种植香根草生产挥发性有机化合物（VOC）的潜力。通过透射电子显微镜研究了根系中的 VOC 积累情况，并通过顶空固相微萃取（HS-SPME）采样后，用气相色谱-质谱联用（GC/MS）分析了这些 VOC 的组成。将 HPA 培养的植物与在盆栽土壤和无菌条件下生长的植物进行了比较。HPA 培养的植物生长不良，根系生物量比在盆栽土壤中生长的植物少。根系细长、更细、更尖，并且缺乏典型的香根草香气。HPA 培养严重损害了通常形成大部分 VOC 的低挥发性碳氢化合物和含氧倍半萜烯的积累。无菌、组织培养的植物也呈现出类似的、更严重的趋势。超微结构分析表明，HPA 条件改变了根系的发生，根中含有较少的 EO 积累细胞，并且含有较少和更不成熟的细胞内 EO 液滴。这些初步结果表明，HPA 培养的香根草发育不良，根系发生紊乱，阻碍了 EO 的积累。
