Evaluation of Immunotropic Activity of Iridoid-Anthocyanin Extract of Honeysuckle Berries ( L.) in the Course of Experimental Trichinellosis in Mice.

Our experiment determined the immunotropic activity of a natural, iridoid-anthocyanin extract from honeysuckle berry ( L.) (LC). The extract was administered to mice infected with , orally at a dose of 2 g/kg bw, six times at 24 h intervals (from day 3 prior to the infection to day 3 post-infection (dpi) with . At 5, 7, 14, and 21 dpi, samples of blood, spleen, and mesenteric lymph nodes (MLN) were collected, and isolated lymphocytes were analyzed by flow cytometry. The splenocyte proliferation was estimated with MTT testing, and the intensity of intestinal and muscle infection was also studied. LC stimulated the local immune system by inducing lymphocyte proliferation in the spleen 7 dpi and altered the percentage and absolute count of B (CD19) and T (CD3, CD8) cells 7, 14, and 21 dpi in the peripheral blood. LC extract affected the dynamics of expulsion of adult from the intestines and prolonged the intestinal phase of the infection but did not change the number of larvae in the muscles. These results suggest that L. fruit extract modulates murine cellular immune response during intestinal phase of infection but shows no antiparasitic activity.