Schäfer Mandy, Pfaff Florian, Höper Dirk, Silaghi Cornelia
Institute of Infectology, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany.
Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany.
Microorganisms. 2022 Feb 28;10(3):525. doi: 10.3390/microorganisms10030525.
Studies on tick-pathogen-host interactions are helping to identify candidates for vaccines against ticks and tick-borne diseases and to discover potent bioactive tick molecules. The tick midgut is the main tissue involved in blood feeding and, moreover, the first organ to have contact with pathogens ingested through the blood meal. As little is known about the molecular biology of feeding and tick defence mechanisms against microorganisms, but important for understanding vector-pathogen interactions, we explored the early transcriptional changes in the midgut of after feeding and in response to challenge with the relapsing-fever spirochete using the Ion S5XL platform. Besides transcripts with metabolic function and immune-related transcripts we discovered numerous putative and uncharacterized protein sequences. Overall, our analyses support previous studies and provides a valuable reference database for further functional proteomic analysis of midgut proteins of .
蜱虫-病原体-宿主相互作用的研究有助于确定抗蜱虫及蜱传疾病疫苗的候选物，并发现具有强大生物活性的蜱虫分子。蜱虫中肠是参与吸血的主要组织，而且是首个接触通过血餐摄入的病原体的器官。由于对蜱虫进食的分子生物学及蜱虫抵御微生物的机制了解甚少，但这对于理解媒介-病原体相互作用很重要，我们利用Ion S5XL平台探索了蜱虫进食后以及用回归热螺旋体攻击后中肠的早期转录变化。除了具有代谢功能的转录本和免疫相关转录本外，我们还发现了许多推定的和未表征的蛋白质序列。总体而言，我们的分析支持了先前的研究，并为进一步对蜱虫中肠蛋白质进行功能蛋白质组学分析提供了有价值的参考数据库。
