Freitas Ana R, Tedim Ana P, Almeida-Santos Ana C, Duarte Bárbara, Elghaieb Houyem, Abbassi Mohamed S, Hassen Abdennaceur, Novais Carla, Peixe Luísa
Laboratory of Microbiology, UCIBIO-Applied Molecular Biosciences Unit, REQUIMTE, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal.
Associate Laboratory i4HB-Institute for Health and Bioeconomy, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal.
Microorganisms. 2022 Mar 16;10(3):632. doi: 10.3390/microorganisms10030632.
Multidrug-resistant (MDR) () infections continue to increase worldwide, although epidemiological studies remain scarce in lower middle-income countries. We aimed to explore which strains circulate in causing human infections in Tunisian healthcare institutions in order to compare them with strains from non-human sources of the same country and finally to position them within the global epidemiology by genomic analysis. Antibiotic susceptibility testing was performed and transfer of vancomycin- and ampicillin- resistance was performed by conjugation. WGS-Illumina was performed on Tunisian strains, and these genomes were compared with genomes from other regions present in the GenBank/NCBI database ( = 10,701 genomes available May 2021). A comparison of phenotypes with those predicted by the recent ResFinder 4.1-CGE webtool unveiled a concordance of 88%, with discordant cases being discussed. cgMLST revealed three clusters [ST18/CT222 ( = 13), ST17/CT948 strains ( = 6), and ST203/CT184 ( = 3)], including isolates from clinical, healthy-human, retail meat, and/or environmental sources in different countries over large time spans (10-12 years). Isolates within each cluster showed similar antibiotic resistance, bacteriocin, and virulence genetic patterns. -AmpR was transferred by VanA-AmpR-ST80 (clinical) and AmpR-ST17- (bovine meat). Identical chromosomal -platforms carrying metabolic/virulence genes were identified between ST17/ST18 strains of clinical, farm animal, and retail meat sources. The overall results emphasize the role of high-resolution genotyping as provided by WGS in depicting the dispersal of MDR- strains carrying relevant adaptive traits across different hosts/regions and the need of a One Health task force to curtail their spread.
多重耐药（MDR）（）感染在全球范围内持续增加，尽管在中低收入国家的流行病学研究仍然很少。我们旨在探索在突尼斯医疗机构中引起人类感染的 菌株，以便将它们与来自同一国家非人类来源的菌株进行比较，并最终通过基因组分析将它们置于全球 流行病学中。进行了抗生素敏感性测试，并通过接合试验进行了万古霉素和氨苄青霉素耐药性的转移。对突尼斯菌株进行了全基因组测序（WGS-Illumina），并将这些基因组与GenBank/NCBI数据库中其他地区的 基因组进行了比较（2021年5月有10701个 基因组可用）。将表型与最近的ResFinder 4.1-CGE网络工具预测的表型进行比较，一致性为88%，对不一致的病例进行了讨论。核心基因组多位点序列分型（cgMLST）揭示了三个聚类[ST18/CT222（n = 13）、ST17/CT948菌株（n = 6）和ST203/CT184（n = 3）]，包括在很长一段时间（10 - 12年）内来自不同国家临床、健康人类、零售肉类和/或环境来源的分离株。每个聚类中的分离株显示出相似的抗生素耐药性、细菌素和毒力基因模式。VanA - AmpR - ST80（临床）和AmpR - ST17 - （牛肉）转移了 - AmpR。在临床、农场动物和零售肉类来源的ST17/ST18菌株之间鉴定出携带代谢/毒力基因的相同染色体 - 平台。总体结果强调了WGS提供的高分辨率基因分型在描绘携带相关适应性状的MDR - 菌株在不同宿主/地区的传播方面的作用，以及需要一个“同一健康”特别工作组来遏制它们的传播。
