Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, 310021 Hangzhou, China.
College of Chemical Engineering, Zhejiang University of Technology, 310014 Hangzhou, China.
Carbohydr Polym. 2022 Jun 15;286:119309. doi: 10.1016/j.carbpol.2022.119309. Epub 2022 Mar 3.
Asteris Radix et Rhizoma (AR) has been widely used as a herbal medicine for treating various symptoms and possesses a number of bioactivities. A neutral polysaccharide ARP-1 was isolated from AR with weight-average molecular weight of 214 kDa. The heteropolysaccharide ARP-1 was composed of fucose, arabinose, galactose, glucose and mannose with a molar ratio of 0.40:14.25:10.22:1.06:0.41. Linkage and NMR analysis showed that ARP-1 had a backbone containing →3,6)-β-d-Galp-(1→ and →6)-β-d-Galp-(1 → residues, and oligosaccharide side chains containing Araf and Galp units were attached to the backbone at C-3 of →3,6)-β-d-Galp-(1 → residues. Antioxidant activity assays showed that ARP-1 exhibited potent antioxidant activities, including ABTS, hydroxy and DPPH radicals scavenging and reducing power. Besides, ARP-1 decreased the production of ROS and MDA, and improved the activities of SOD, which resulted in the protection of PC12 cells against HO-induced oxidative stress. The findings indicated that ARP-1 might be used as a potential natural antioxidant.
紫菀根（Asteris Radix et Rhizoma，AR）作为一种草药被广泛应用于治疗各种症状，并具有多种生物活性。一种中性多糖 ARP-1 从紫菀中分离得到，其重均分子量为 214 kDa。该杂多糖 ARP-1 由岩藻糖、阿拉伯糖、半乳糖、葡萄糖和甘露糖组成，摩尔比为 0.40:14.25:10.22:1.06:0.41。连接和 NMR 分析表明，ARP-1 具有含有→3,6)-β-d-Galp-(1→和→6)-β-d-Galp-(1→残基的主链，以及以 C-3 连接到主链上的含有 Araf 和 Galp 单元的寡糖侧链。抗氧化活性测定表明，ARP-1 具有很强的抗氧化活性，包括 ABTS、羟基和 DPPH 自由基清除能力和还原能力。此外，ARP-1 降低了 ROS 和 MDA 的产生，提高了 SOD 的活性，从而保护 PC12 细胞免受 HO 诱导的氧化应激。研究结果表明，ARP-1 可能作为一种潜在的天然抗氧化剂。
