College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Tai'an, 271018, Shandong, PR China.
Department of Biological and Agricultural Engineering, University of California, Davis, 95616, CA, USA; Research and Development, The Morning Star Company, Woodland, 95695, CA, USA.
Carbohydr Polym. 2021 Jun 1;261:117879. doi: 10.1016/j.carbpol.2021.117879. Epub 2021 Mar 1.
A neutral polysaccharide (HJP-1a) and three acid polysaccharides (HJP-2, HJP-3 and HJP-4) were obtained from Z. jujuba cv. Hamidazao. HJP-1a was mainly composed of arabinose and galactose in a ratio of 56.9:20.0, with an average molecular weight of 3.115 × 10 g/mol. HJP-2, HJP-3 and HJP-4 were homogeneous heteropolysaccharides mainly containing galacturonic acid, arabinose and galactose, with average molecular weights of 4.590 × 10, 6.986 × 10 and 1.951 × 10 g/mol, respectively. Structural characterization indicated that the backbone of HJP-3 appeared to be mainly composed of →4)-α-d-GalpA (1→ and →2,4)-α-l-Rhap (1→ residues with some branches consisting of →5)-α-l-Araf (1→ residues and terminals of T-α-l-Araf (1→ and T-β-d-Galp residues. The four purified fractions displayed dose-dependent radical scavenging activity on ABTS radicals and reducing capacity, as well as excellent protective effect on HO-induced HepG2 cells and metronidazole-damaged zebrafish embryos, especially HJP-2 in vitro and HJP-1a in vivo. Therefore, the polysaccharides from Z. jujuba cv. Hamidazao could be used as a potential antioxidant in functional foods.
从酸枣品种哈蜜早中得到一种中性多糖（HJP-1a）和三种酸性多糖（HJP-2、HJP-3 和 HJP-4）。HJP-1a 主要由阿拉伯糖和半乳糖组成，比例为 56.9:20.0，平均分子量为 3.115×10 g/mol。HJP-2、HJP-3 和 HJP-4 均为均一的杂多糖，主要含有半乳糖醛酸、阿拉伯糖和半乳糖，平均分子量分别为 4.590×10、6.986×10 和 1.951×10 g/mol。结构表征表明 HJP-3 的糖链骨架主要由→4)-α-d-GalpA（1→和→2,4）-α-l-Rhap（1→残基组成，带有一些由→5）-α-l-Araf（1→残基和 T-α-l-Araf（1→和 T-β-d-Galp 末端组成的支链。四个纯化的部分对 ABTS 自由基和还原能力均表现出剂量依赖性的自由基清除活性，以及对 HO 诱导的 HepG2 细胞和甲硝唑损伤的斑马鱼胚胎的优异保护作用，尤其是 HJP-2 在体外和 HJP-1a 在体内。因此，酸枣品种哈蜜早中的多糖可作为功能性食品中的潜在抗氧化剂。
