College of arts and sciences, Shanxi Agricultural University, Taigu, 030801, Shanxi, China.
College of chemistry and pharmacy, Northwest Agricultural and Forestry University, Yangling, 712100, Shaanxi, China.
Glycoconj J. 2020 Jun;37(3):361-371. doi: 10.1007/s10719-020-09916-0. Epub 2020 Mar 5.
Radix Paeoniae Alba is widely used in Chinese traditional medicine to treat various diseases such as gastrointestinal disorders, immunomodulatory, cancer, and other diseases. In this paper, a novel acidic polysaccharide RPAPS purified from Radix Paeoniae Alba was evaluated for its structural features and potential of immunomodulatory and antioxidant activities. RPAPS (molecular weight: 1.0× 10 Da) was mainly composed of α-(1 → 4)-Glcp, α-Arap, α-Galp, α-Rhap, β-D-Glcp, α-(1 → 6)-linked Glcp and GalA. Immunological tests indicated that RPAPS could improve RAW264.7 phagocytic activity and LPS-induced splenocyte proliferation. For antioxidant activities, RPAPS showed reducing power and DPPH scavenging activity in dose dependent. Moreover, RPAPS could significantly protect the PC12 cells from HO damage. These data implied polysaccharides RPAPS had the potential to be novel natural antioxidative and immunopotentiating agents for using in functional foods or medicine.
白芍被广泛应用于中医治疗各种疾病，如胃肠道疾病、免疫调节、癌症等。在本文中，我们评价了一种从白芍中提取的新型酸性多糖 RPAPS 的结构特征及其免疫调节和抗氧化活性。RPAPS（分子量：1.0×105Da）主要由α-(1→4)-Glcp、α-Arap、α-Galp、α-Rhap、β-D-Glcp、α-(1→6)-连接的 Glcp 和 GalA 组成。免疫试验表明，RPAPS 可提高 RAW264.7 吞噬细胞的吞噬活性和 LPS 诱导的脾细胞增殖。抗氧化活性试验表明，RPAPS 具有剂量依赖性的还原力和 DPPH 清除活性。此外，RPAPS 可显著保护 PC12 细胞免受 H2O2 损伤。这些数据表明，多糖 RPAPS 具有成为新型天然抗氧化和免疫增强剂的潜力，可用于功能性食品或药物。
