接种灭活的严重急性呼吸综合征冠状病毒 2 型（SARS-CoV-2）疫苗（科兴疫苗）和/或 SARS-CoV-2 感染对抗体水平、浆母细胞、长寿浆细胞和自然杀伤细胞产生干扰素-γ的影响。
The effect of immunization with inactivated SARS-CoV-2 vaccine (CoronaVac) and/or SARS-CoV-2 infection on antibody levels, plasmablasts, long-lived-plasma-cells, and IFN-γ release by natural killer cells.
机构信息
Marmara University, School of Medicine, Department of Infectious Diseases and Clinical Microbiology, Istanbul, Turkey.
Marmara University, School of Medicine, Department of Infectious Diseases and Clinical Microbiology, Istanbul, Turkey.
出版信息
Vaccine. 2022 Apr 20;40(18):2619-2625. doi: 10.1016/j.vaccine.2022.03.001. Epub 2022 Mar 18.
OBJECTIVES
We evaluated the antibody response, natural killer cell response and B cell phenotypes in healthcare workers (HCW) who are vaccinated with two doses of CoronaVac with or without documented SARS-CoV-2 infection and unvaccinated HCWs with SARS-CoV-2 infection.
METHODS
HCWs were divided into four groups: vaccine only (VO), vaccine after SARS-CoV-2 infection (VAI), SARS-CoV-2 infection only (IO), and SARS-CoV-2 infection after vaccine (IAV). Anti-SARS-CoV-2 spike protein (Anti-S) antibodies were measured by Elecsys Anti-SARS-CoV-2 S ELISA kit. Memory B cells (CD19CD27), plasmablast B cells (CD19CD138) and long-lived plasma cells (LLPC; CD138CD19) were measured by flow cytometry in 74 patients. Interferon gamma (IFN-γ) release by natural killer (NK) cells were measured by NKVue Test (NKMAX, Republic of Korea) in 76 patients. RT-PCR was performed with Bio-speedy® COVID-19 qPCR detection kit, Version 2 (Bioexen LTD, Istanbul, Turkey).
RESULTS
The Anti-S antibodies were detectable in all HCWs (n: 224). The median Anti-S titers (BAU/mL) was significantly higher in VAI (620 25-75% 373-1341) compared to VO (136, 25-75% 85-283) and IO (111, 25-75% 54-413, p < 0.01). VAI group had significantly lower percentage of plasmablasts (2.9; 0-8.7) compared to VO (6.8; 3.5-12.0) and IO (9.9; 4.7-47.5, p < 0.01) (n:74). Percentage of LLPCs in groups VO, VAI and IO was similar. There was no difference of IFN-γ levels between the study groups (n: 76).
CONCLUSION
The antibody response was similar between uninfected vaccinated HCWs and unvaccinated HCWs who had natural infection. HCWs who had two doses of CoronaVac either before or after the natural SARS-CoV-2 infection elicited significantly higher antibody responses compared to uninfected vaccinated HCWs. The lower percentages of plasmablasts in the VAI group may indicate their migration to lymph nodes and initiation of the germinal center reaction phase. IFN-γ response did not differ among the groups.
目的
评估接种两剂科兴疫苗（CoronaVac）的医护人员（HCW）和接种过疫苗且有记录的 SARS-CoV-2 感染史的医护人员（VAI）、仅感染 SARS-CoV-2 的医护人员（IO）以及感染 SARS-CoV-2 后接种疫苗的医护人员（IAV）的抗体反应、自然杀伤（NK）细胞反应和 B 细胞表型。
方法
将 HCW 分为四组：仅接种疫苗（VO）、接种疫苗后感染 SARS-CoV-2（VAI）、仅感染 SARS-CoV-2（IO）和感染 SARS-CoV-2 后接种疫苗（IAV）。使用 Elecsys Anti-SARS-CoV-2 S ELISA 试剂盒检测抗 SARS-CoV-2 刺突蛋白（Anti-S）抗体。在 74 名患者中通过流式细胞术测量记忆 B 细胞（CD19CD27）、浆母细胞（CD19CD138）和长寿浆细胞（LLPC；CD138CD19）。在 76 名患者中使用 NKVue Test（NKMAX，韩国共和国）测量 NK 细胞产生的干扰素（IFN-γ）。使用 Bio-speedy® COVID-19 qPCR 检测试剂盒，版本 2（Bioexen LTD，土耳其伊斯坦布尔）进行 RT-PCR。
结果
所有 HCW（n=224）均检测到 Anti-S 抗体。与 VO（136，25-75%85-283）和 IO（111，25-75%54-413）相比，VAI（620，25-75%373-1341）的 Anti-S 滴度（BAU/mL）中位数显著更高（p<0.01）。与 VO（6.8，3.5-12.0）和 IO（9.9，4.7-47.5）相比，VAI 组的浆母细胞百分比（2.9，0-8.7）显著降低（p<0.01）（n=74）。VO、VAI 和 IO 组的 LLPC 百分比相似。各组之间 IFN-γ 水平无差异（n=76）。
结论
未感染接种疫苗的 HCW 与自然感染的未接种疫苗的 HCW 之间的抗体反应相似。与未感染接种疫苗的 HCW 相比，接种两剂科兴疫苗（CoronaVac）的 HCW 无论是在自然感染之前还是之后，均产生了更高的抗体反应。VAI 组浆母细胞百分比较低，可能表明其迁移至淋巴结并启动生发中心反应阶段。IFN-γ 反应在各组之间无差异。
Zotero 插件