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用于检测人抗弓形虫免疫球蛋白M抗体的快速双夹心酶联免疫吸附测定法。

Rapid double-sandwich enzyme-linked immunosorbent assay for detection of human immunoglobulin M anti-Toxoplasma gondii antibodies.

作者信息

Tomasi J P, Schlit A F, Stadtsbaeder S

出版信息

J Clin Microbiol. 1986 Nov;24(5):849-50. doi: 10.1128/jcm.24.5.849-850.1986.

Abstract

The double-sandwich enzyme-linked immunosorbent assay has been compared with the indirect fluorescence assay for the detection of immunoglobulin M antibodies against Toxoplasma gondii in humans. Incubation times have been shortened, permitting the test to be completed within 2 h. The double-sandwich enzyme-linked immunosorbent assay is confirmed to be more sensitive and more specific than the immunofluorescence assay.

摘要

已将双夹心酶联免疫吸附测定法与间接荧光测定法进行比较,以检测人体中抗弓形虫的免疫球蛋白M抗体。孵育时间已缩短,使得该测试能在2小时内完成。证实双夹心酶联免疫吸附测定法比免疫荧光测定法更敏感、更特异。

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