Payne R A, Isaac M, Francis J M
J Clin Pathol. 1982 Aug;35(8):892-6. doi: 10.1136/jcp.35.8.892.
Sera from 180 patients with suspected toxoplasmic lymphadenopathy were examined for antitoxoplasma IgM by an enzyme-linked immunosorbent assay (ELISA), using antibody class capture (ACCA). Of 82 positive ACCA results, 78 were confirmed by testing the IgM fractions of the sera, obtained by sucrose density gradient centrifugation (SDGC). The four positive results which could not be confirmed were all from patients with at least a year's history of lymphadenopathy. Sera from 10 patients with low Sabin Feldman dye test (DT) titers gave positive ACCA results and subsequent specimens from them showed a rise in antibody concentration, confirming the diagnosis of acute toxoplasmosis. The antitoxoplasma IgM immunofluorescent antibody test (IgM-IFA) on whole serum was relatively insensitive and gave false-positive results with sera containing rheumatoid factor (RF) and antinuclear factor (ANF). There were no false-positive ACCA results with such sera, probably because the conjugates were prepared from F(ab')2 fragments of antitoxoplasma serum. The ACCA proved to be sensitive, specific and easily automated enabling examination of large numbers of specimens.
采用抗体类别捕获法（ACCA），通过酶联免疫吸附测定（ELISA）对180例疑似弓形虫淋巴结病患者的血清进行抗弓形虫IgM检测。在82例ACCA检测结果呈阳性的患者中，78例通过检测经蔗糖密度梯度离心（SDGC）获得的血清IgM组分得以确诊。4例无法确诊的阳性结果均来自淋巴结病病史至少一年的患者。10例Sabin Feldman染色试验（DT）滴度较低的患者血清ACCA检测结果呈阳性，随后采集的样本显示抗体浓度升高，从而确诊为急性弓形虫病。对全血清进行抗弓形虫IgM免疫荧光抗体检测（IgM-IFA）相对不敏感，且对含有类风湿因子（RF）和抗核因子（ANF）的血清会给出假阳性结果。此类血清ACCA检测未出现假阳性结果，可能是因为结合物是由抗弓形虫血清的F(ab')2片段制备而成。事实证明，ACCA检测灵敏、特异且易于自动化，能够对大量样本进行检测。
