Hébert G A, Penner J L, Hennessy J N, McKinney R M
J Clin Microbiol. 1983 Nov;18(5):1064-9. doi: 10.1128/jcm.18.5.1064-1069.1983.
Rabbits were inoculated with whole, formalinized cells from eight passive hemagglutination reference strains of Campylobacter. Fluorescein isothiocyanate-labeled immunoglobulin G from these antisera defined seven new direct fluorescent-antibody serogroups of C. jejuni and one new serogroup of C. coli. This expanded the Campylobacter direct fluorescent antibody system to include 17 serogroups of C. jejuni, 3 serogroups of C. coli, and 2 serogroups of C. fetus. We then compared the passive hemagglutination method (57 serotypes) and the direct fluorescent-antibody method (20 serogroups) for typing strains of C. jejuni and C. coli. The data obtained by testing 101 strains by both methods revealed that the two test systems were measuring completely different sets of antigen complexes. The two serogrouping methods were complementary, and their combined use discriminated among strains more effectively than did either method individually.
用来自弯曲杆菌八个被动血凝参考菌株的完整、经福尔马林固定的细胞对兔子进行接种。这些抗血清中异硫氰酸荧光素标记的免疫球蛋白G确定了空肠弯曲菌的七个新的直接荧光抗体血清群和结肠弯曲菌的一个新血清群。这将弯曲杆菌直接荧光抗体系统扩展到包括空肠弯曲菌的17个血清群、结肠弯曲菌的3个血清群和胎儿弯曲菌的2个血清群。然后，我们比较了被动血凝法（57种血清型）和直接荧光抗体法（20个血清群）对空肠弯曲菌和结肠弯曲菌菌株进行分型的情况。通过两种方法对101株菌株进行检测所获得的数据表明，这两种检测系统测量的是完全不同的抗原复合物组。这两种血清分组方法是互补的，与单独使用任何一种方法相比，它们联合使用能更有效地区分不同菌株。
