LBPS/CSPBAT, UMR CNRS 7244, Institut Galilée, Université Sorbonne Paris Nord, 99 avenue JB Clément, 93430 Villetaneuse, France.
LBPS/CSPBAT, UMR CNRS 7244, Institut Galilée, Université Sorbonne Paris Nord, 99 avenue JB Clément, 93430 Villetaneuse, France.
Biophys Chem. 2022 Jun;285:106804. doi: 10.1016/j.bpc.2022.106804. Epub 2022 Mar 23.
Proteins adsorption occurs spontaneously on biomaterial upon insertion within the body. The resulting protein layer influences biomaterial biocompatibility through enhanced bio-integration or, on the contrary, adverse reactions. Furthermore, upon adsorption, proteins can undergo modifications of their structure and, ultimately, their physicochemical properties and activity. Hence, the understanding of protein adsorption on implanted materials appears essential, as exemplified by silicone breast prostheses that might lead to serious health issues. Surface modifications with a bioactive polymer, poly(styrene sodium sulfonate)-polyNaSS, on a hydrophobic silicone surface that composes breast implants, have been successfully performed under UV irradiation by a radical surface polymerization. This strategy enhances cell biocompatibility and antibacterial features. Although detailed insights related to the mechanism are still scarce, polyNaSS is supposed to promote changes in the conformation and/or orientation of adsorbed plasma proteins, reducing the odd for a biofilm to form. The present work addresses more in-depth structural investigations of the adsorbed state of two plasma proteins: Bovine Serum Albumin (BSA), as a model protein, and fibronectin (FN), for its role in cell adhesion. Using Atomic force microscopy (AFM), we report that polyNaSS showed no significant impact on the BSA structure conversely to the FN one. However, imaging findings with AFM clearly outlined a change in the structural organization of FN, going from a nano fibrillar assembly with an average length of 130 nm to a globular one when the surface was grafted. Thus, it is highlighted that polyNaSS interacts specifically with FN. In addition, cell spreading assay of L929 fibroblasts on FN-coated surfaces with optical microscopy indicated no significant impact of the change in FN structure upon fibroblasts adhesion, which displayed active elongated shapes. The present features are crucial for understanding the cell adhesion mechanism induced by surface modification.
蛋白质在体内插入生物材料时会自发吸附在生物材料上。由此产生的蛋白质层通过增强生物整合或相反的不良反应来影响生物材料的生物相容性。此外，蛋白质在吸附后可以改变其结构，最终改变其物理化学性质和活性。因此，理解蛋白质在植入材料上的吸附显得至关重要，例如硅酮乳房假体可能会导致严重的健康问题。通过自由基表面聚合，在组成乳房植入物的疏水性硅酮表面上用生物活性聚合物聚（苯乙烯磺酸钠）-聚 NaSS 进行表面改性已经在 UV 照射下成功进行。该策略提高了细胞生物相容性和抗菌性能。尽管与机制相关的详细见解仍然很少，但聚 NaSS 被认为可以促进吸附血浆蛋白的构象和/或取向的变化，减少生物膜形成的可能性。本工作更深入地研究了两种血浆蛋白的吸附状态：牛血清白蛋白（BSA）作为模型蛋白，以及纤维连接蛋白（FN）作为细胞黏附的作用。使用原子力显微镜（AFM），我们报告说聚 NaSS 对 BSA 结构没有显著影响，而 FN 则没有。然而，AFM 的成像结果清楚地描绘了 FN 结构组织的变化，从具有平均长度为 130nm 的纳米纤维组装体到接枝表面时的球形。因此，聚 NaSS 与 FN 特异性相互作用。此外，光学显微镜下 L929 成纤维细胞在 FN 涂层表面上的细胞扩展实验表明，FN 结构的变化对成纤维细胞黏附没有显著影响，成纤维细胞显示出活跃的伸长形状。这些特征对于理解表面改性诱导的细胞黏附机制至关重要。
