Muraoka Satoshi, Hirano Masayo, Isoyama Junko, Nagayama Satoshi, Tomonaga Takeshi, Adachi Jun
Laboratory of Proteome Research, National Institute of Biomedical Innovation, Health and Nutrition, 7-6-8, Saito-Asagi, Ibaraki City, Osaka 567-0085, Japan.
Laboratory of Proteomics for Drug Discovery, Center for Drug Design Research, National Institute of Biomedical Innovation, Health and Nutrition, Osaka 567-0085, Japan.
iScience. 2022 Mar 1;25(4):104012. doi: 10.1016/j.isci.2022.104012. eCollection 2022 Apr 15.
Extracellular vesicles (EVs) are ubiquitously secreted by almost all tissues and carry many cargoes, including proteins, RNAs, and lipids, which are related to various biological processes. EVs are shed from tissues into the blood and expected to be used as biomarkers for diseases. Here, we isolated EVs from EDTA plasma and serum of six healthy subjects by an affinity capture isolation method, and a total of 4,079 proteins were successfully identified by comprehensive EV proteomics. Our reliable and detailed catalog of the differential expression profiles of EV proteins in plasma and serum between healthy individuals could be useful as a reference for biomarker discovery. Furthermore, tissue-specific protein groups co-regulated between blood EVs from healthy individuals were identified. These EV proteins are expected to be used for more specific and sensitive enrichment of tissue-specific EVs and for screening and monitoring of disease without diagnostic imaging in patient blood in the future.
细胞外囊泡（EVs）几乎由所有组织普遍分泌，并携带许多货物，包括与各种生物过程相关的蛋白质、RNA和脂质。EVs从组织中释放到血液中，并有望用作疾病的生物标志物。在这里，我们通过亲和捕获分离方法从六名健康受试者的乙二胺四乙酸（EDTA）血浆和血清中分离出EVs，并通过全面的EV蛋白质组学成功鉴定了总共4079种蛋白质。我们可靠且详细的健康个体血浆和血清中EV蛋白质差异表达谱目录可作为生物标志物发现的参考。此外，还鉴定了健康个体血液EVs之间共同调节的组织特异性蛋白质组。这些EV蛋白质有望在未来用于更特异性和敏感地富集组织特异性EVs，并用于在患者血液中无需诊断成像的疾病筛查和监测。
