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黑色素瘤患者血浆中黑素瘤细胞衍生的小细胞外囊泡的蛋白质组学特征：黑色素瘤进展的潜在相关物。

Proteomic profile of melanoma cell-derived small extracellular vesicles in patients' plasma: a potential correlate of melanoma progression.

机构信息

Maria Sklodowska-Curie National Research Institute of Oncology Gliwice Poland.

European Center for Bioinformatics and Genomics Institute of Bioorganic Chemistry PAS Poznan Poland.

出版信息

J Extracell Vesicles. 2021 Feb;10(4):e12063. doi: 10.1002/jev2.12063. Epub 2021 Feb 11.

DOI:10.1002/jev2.12063
PMID:33613873
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7876545/
Abstract

Molecular profiling of small extracellular vesicles (sEV) isolated from plasma of cancer patients emerges as promising strategy for biomarkers discovery. We investigated the proteomic profiles of sEV immunoselected using anti-CSPG4 antibodies from 15 melanoma patients' plasma. The proteomes of sEV separated into melanoma cell-derived (MTEX) and non-malignant cell-derived (NMTEX) were compared using high-resolution mass spectrometry. Paired analysis identified the MTEX-associated profile of 16 proteins that discriminated MTEX from NMETEX. We also identified the MTEX profile that discriminated between seven patients with no evidence of melanoma (NED) after therapy and eight with progressive disease (PD). Among 75 MTEX proteins overexpressed in PD patients, PDCD6IP (ALIX) had the highest discriminating value, while CNTN1 (contactin-1) was upregulated only in MTEX of NED patients. This is the first report documenting that proteomes of tumour-derived sEV in patients' plasma discriminate cancer from non-cancer and identify proteins with potential to serve as prognostic biomarkers in melanoma.

摘要

从癌症患者血浆中分离出的小细胞外囊泡 (sEV) 的分子特征分析显示出作为生物标志物发现的有前途的策略。我们研究了使用抗 CSPG4 抗体从 15 名黑色素瘤患者的血浆中免疫分离的 sEV 的蛋白质组特征。使用高分辨率质谱法比较了 sEV 分离的黑色素瘤细胞衍生的 (MTEX) 和非恶性细胞衍生的 (NMTEX) 的蛋白质组。配对分析确定了 16 种蛋白质的 MTEX 相关特征，可将 MTEX 与 NMETEX 区分开来。我们还确定了在治疗后无黑色素瘤证据 (NED) 的 7 名患者和进展性疾病 (PD) 的 8 名患者之间的 MTEX 特征。在 PD 患者中过表达的 75 种 MTEX 蛋白中，PDCD6IP (ALIX) 具有最高的区分价值，而 CNTN1（接触蛋白-1）仅在 NED 患者的 MTEX 中上调。这是第一个记录肿瘤衍生的 sEV 在患者血浆中的蛋白质组可区分癌症和非癌症，并确定具有作为黑色素瘤预后生物标志物潜力的蛋白质的报告。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ef3/7876545/cf4af9e5cd76/JEV2-10-e12063-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ef3/7876545/1672b788e469/JEV2-10-e12063-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ef3/7876545/34d2f87f3d63/JEV2-10-e12063-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ef3/7876545/b725887f6a30/JEV2-10-e12063-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ef3/7876545/3d77de9dbcb6/JEV2-10-e12063-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ef3/7876545/d1e80e319f22/JEV2-10-e12063-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ef3/7876545/cf4af9e5cd76/JEV2-10-e12063-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ef3/7876545/1672b788e469/JEV2-10-e12063-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ef3/7876545/34d2f87f3d63/JEV2-10-e12063-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ef3/7876545/b725887f6a30/JEV2-10-e12063-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ef3/7876545/3d77de9dbcb6/JEV2-10-e12063-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ef3/7876545/d1e80e319f22/JEV2-10-e12063-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ef3/7876545/cf4af9e5cd76/JEV2-10-e12063-g006.jpg

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