一种对人朗格汉斯细胞具有特异性反应的单克隆抗体。

A monoclonal antibody specifically reactive to human Langerhans cells.

作者信息

Kashihara M, Ueda M, Horiguchi Y, Furukawa F, Hanaoka M, Imamura S

出版信息

J Invest Dermatol. 1986 Nov;87(5):602-7. doi: 10.1111/1523-1747.ep12455849.

Abstract

We prepared a mouse monoclonal antibody that reacts specifically to human Langerhans cells (LC). The protein recognized by this antibody was mainly in the membranes of Birbeck granules and related structures. Using this antibody, we could identify LC in various tissues; these cells were in the skin, stratified squamous mucosal epithelia, lymph nodes, and the thymus. The antibody did not react with monocytes, tissue macrophages, lymphoid dendritic cells, follicular dendritic cells, or interdigitating cells. The antigen purified with this antibody was a heterogeneously glycosylated protein of Mr approximately 40,000 without interchain disulfide bonds. This antibody may be useful for identifying LC in various human tissues with or without abnormalities, and for studying the origin and fate of Birbeck granules of LC.

摘要

我们制备了一种小鼠单克隆抗体，它能特异性地与人朗格汉斯细胞（LC）发生反应。该抗体识别的蛋白主要存在于伯贝克颗粒及相关结构的膜上。利用这种抗体，我们能够在各种组织中识别LC；这些细胞存在于皮肤、复层鳞状黏膜上皮、淋巴结和胸腺中。该抗体不与单核细胞、组织巨噬细胞、淋巴样树突状细胞、滤泡树突状细胞或交错突细胞发生反应。用这种抗体纯化的抗原是一种Mr约为40,000的异质性糖基化蛋白，无链间二硫键。这种抗体可能有助于在各种正常或异常的人体组织中识别LC，并用于研究LC伯贝克颗粒的起源和命运。