School and Hospital of Stomatology, Kunming Medical University, Kunming, China.
Department of Orthodontics, Affiliated Hospital of Yunnan University, Kunming, China.
Cells Tissues Organs. 2023;212(4):317-326. doi: 10.1159/000524291. Epub 2022 Mar 28.
Periodontal ligament stem cells (PDLSCs) possess self-renewal and multilineage differentiation potential and exhibit great potential for the treatment of bone tissue defects caused by inflammation. Previous studies have indicated that static magnetic field (SMF) can enhance the proliferation and differentiation of mesenchymal stem cells (MSCs). SMF has been widely used to repair bone defects and for orthodontic and implantation treatment. In this study, we revealed that a 320 mT SMF upregulates the protein expression levels of cytokines such as MCM7 and PCNA in proliferating PDLSCs. Cell counting kit-8 results revealed that the SMF group had higher optical density values than the control group. The ratio of cells in the S phase to those in the G2/M phase was significantly increased after exposure to a 320 mT SMF. In scratch assays, the SMF-treated PDLSCs exhibited a higher migration rate than the sham-exposed group after 24 h of culture, indicating that the SMF promoted the migratory ability of PDLSCs. The activity level of the early differentiation marker alkaline phosphatase and the late marker matrix mineralization, as well as osteoblast-specific gene and protein expression, were enhanced in PDLSCs exposed to the SMF. Furthermore, AKT signaling pathway was activated by SMF. Our data demonstrated that the potential mechanism of action of SMF may enhance PDLSCs proliferation and osteogenic differentiation by activating the phosphorylated AKT pathway. The elucidation of this molecular mechanism may lead to a better understanding of bone repair responses and aid in improved stem cell-mediated regeneration.
牙周膜干细胞（PDLSCs）具有自我更新和多向分化潜能，在治疗炎症引起的骨组织缺损方面具有巨大潜力。先前的研究表明，静磁场（SMF）可以增强间充质干细胞（MSCs）的增殖和分化。SMF 已被广泛用于修复骨缺损以及正畸和植入物治疗。在这项研究中，我们揭示了 320 mT 的 SMF 上调了增殖的 PDLSCs 中细胞周期蛋白 MCM7 和 PCNA 等细胞因子的蛋白表达水平。细胞计数试剂盒-8 的结果显示，SMF 组的光密度值高于对照组。暴露于 320 mT 的 SMF 后，S 期细胞与 G2/M 期细胞的比例显著增加。划痕实验结果显示，SMF 处理的 PDLSCs 在培养 24 小时后迁移率高于假暴露组，表明 SMF 促进了 PDLSCs 的迁移能力。碱性磷酸酶（早期分化标志物）和基质矿化（晚期标志物）的活性水平以及成骨细胞特异性基因和蛋白表达在暴露于 SMF 的 PDLSCs 中增强。此外，SMF 激活了 AKT 信号通路。我们的数据表明，SMF 的潜在作用机制可能通过激活磷酸化 AKT 通路来增强 PDLSCs 的增殖和成骨分化。阐明这一分子机制可能有助于更好地理解骨修复反应，并有助于改善基于干细胞的再生。
