Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK.
Genome Biol. 2022 Mar 28;23(1):85. doi: 10.1186/s13059-022-02652-8.
Determining the evolutionary relationships between genes is fundamental to comparative biological research. Here, we present SHOOT. SHOOT searches a user query sequence against a database of phylogenetic trees and returns a tree with the query sequence correctly placed within it. We show that SHOOT performs this analysis with comparable speed to a BLAST search. We demonstrate that SHOOT phylogenetic placements are as accurate as conventional tree inference, and it can identify orthologs with high accuracy. In summary, SHOOT is a fast and accurate tool for phylogenetic analyses of novel query sequences. It is available online at www.shoot.bio .
确定基因之间的进化关系是比较生物学研究的基础。在这里，我们介绍 SHOOT。SHOOT 会将用户查询序列与系统中的系统发育树数据库进行比对，并返回一个将查询序列正确定位的树。我们表明，SHOOT 的分析速度与 BLAST 搜索相当。我们还证明，SHOOT 的系统发育定位与传统的树推断一样准确，并且可以高精度地识别同源基因。总的来说，SHOOT 是一种快速准确的新查询序列系统发育分析工具。它可在www.shoot.bio 上在线使用。
