Zuo Shuting, Wang Jing, An Xianquan, Zhang Yan
Department of Breast Surgery, The Second Hospital of Jilin University, Changchun, China.
Department of Anesthesiology, The Second Hospital of Jilin University, Changchun, China.
Front Bioeng Biotechnol. 2022 Mar 8;9:763486. doi: 10.3389/fbioe.2021.763486. eCollection 2021.
Protein therapeutics have been considered a promising strategy for cancer treatment due to their highly specific bioactivity and few side effects. Unfortunately, the low physiological stability and poor membrane permeability of most protein drugs greatly limit their clinical application. Furthermore, single-modality protein therapeutics show insufficient efficacy. To address these issues, Janus magnetic mesoporous silica nanoparticles (Janus MSNNPs) were developed to preload ribonuclease A (RNaseA) to simultaneously realize the magnetically enhanced delivery of protein drugs and magnetic hyperthermia-enhanced protein therapy. Janus MSNNPs showed a high RNaseA loading ability and pH-responsive drug release behavior. Furthermore, an external magnetic field could remarkably enhance the therapeutic effect of RNaseA-loaded Janus MSNNPs due to the improved intracellular internalization of RNaseA. Importantly, Janus MSNNPs possessed an outstanding magnetic hyperthermia conversion efficiency, which could generate hyperthermia under an alternating magnetic field, effectively supplementing protein therapy by a combined effect. and experiments confirmed the high anticancer outcome and low side effects of this intriguing strategy for breast cancer based on Janus MSNNPs. Hence, Janus MSNNPs might be an effective and safe nanoplatform for magnetically combined protein therapy.
由于蛋白质疗法具有高度特异性的生物活性和较少的副作用，它已被视为一种有前景的癌症治疗策略。不幸的是，大多数蛋白质药物的生理稳定性低和膜通透性差极大地限制了它们的临床应用。此外，单模态蛋白质疗法显示出疗效不足。为了解决这些问题，开发了双面磁性介孔二氧化硅纳米颗粒（Janus MSNNPs）来预载核糖核酸酶A（RNaseA），以同时实现蛋白质药物的磁增强递送和磁热增强蛋白质治疗。Janus MSNNPs表现出高RNaseA负载能力和pH响应性药物释放行为。此外，由于RNaseA细胞内内化的改善，外部磁场可以显著增强负载RNaseA的Janus MSNNPs的治疗效果。重要的是，Janus MSNNPs具有出色的磁热转换效率，可在交变磁场下产生热疗，通过联合作用有效补充蛋白质治疗。细胞实验和动物实验证实了这种基于Janus MSNNPs的有趣的乳腺癌治疗策略具有高抗癌效果和低副作用。因此，Janus MSNNPs可能是一种用于磁联合蛋白质治疗的有效且安全的纳米平台。
