Department of Endodontics, Mashhad University of Medical Sciences, Faculty of Dentistry, Mashhad, Iran.
Department of Endodontics, Private Practice, Mashhad, Iran.
Eur Endod J. 2022 Mar;7(1):67-72. doi: 10.14744/eej.2021.04696.
Enterococcus faecalis (E. faecalis) is one of the persistent microorganisms responsible for the failure of root canal treatments. This study investigated the antibacterial property, the time-killing of 3 peptides, namely Tachyplesin, Thanatin, and Enterocin P, on E. faecalis.
In this study, recombinant peptides were synthesized via secretory synthesis. The peptides were then purified and isolated using affinity chromatography after which their purification was evaluated through SDS-PAGE. The antimicrobial activity of peptides against E. faecalis was tested using the minimum inhibitory concentration test (MIC), the minimum bactericidal concentration test (MBC), and the time-killing assay.
Based on antimicrobial tests, a similar value was observed for the MIC and MBC in the recombinant peptide of Enterocin P. The concentration of MBC was twice as much as that of MIC for Tanatin and Tachyplesin. The time-killing-assay antimicrobial test showed that Enterocin P has a better pattern and antimicrobial activity than the other two peptides; all three peptides have weaker antimicrobial activities than sodium hypochlorite.
Considering the equivalence of MIC and MBC in the recombinant peptide of Enterocin P, it can be a viable replacement for traditional disinfectants and medicaments used in root canal treatment procedures.
粪肠球菌（E. faecalis）是导致根管治疗失败的持久性微生物之一。本研究调查了 3 种肽，即 tachyplesin、thanatin 和 enterocin P 对粪肠球菌的抗菌特性和杀菌时间。
在本研究中，通过分泌合成合成了重组肽。然后通过亲和层析对肽进行纯化和分离，并用 SDS-PAGE 评估其纯度。使用最低抑菌浓度试验（MIC）、最低杀菌浓度试验（MBC）和杀菌时间试验测试肽对粪肠球菌的抗菌活性。
根据抗菌试验，重组 Enterocin P 肽的 MIC 和 MBC 值相似。Tanatin 和 Tachyplesin 的 MBC 浓度是 MIC 的两倍。杀菌时间试验抗菌试验表明，Enterocin P 的杀菌效果优于另外两种肽；三种肽的抗菌活性均弱于次氯酸钠。
考虑到重组 Enterocin P 肽的 MIC 和 MBC 等效，它可以替代根管治疗程序中传统的消毒剂和药物。
