Tanhaieian Abbas, Pourgonabadi Solmaz, Akbari Majid, Mohammadipour Hamideh-Sadat
Dental research center, School of Dentistry, Mashhad University of Medical Sciences Mashhad, Iran.
Oral and maxillofacial department, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran.
J Clin Exp Dent. 2020 Jun 1;12(6):e523-e532. doi: 10.4317/jced.55717. eCollection 2020 Jun.
This study was conducted aimed at evaluating the antibacterial property of the recombinant peptide of bacteriocin entrocin P (EnP), the essential oil of Cuminum cyminum, and the extract of on some oral pathogens. Besides, the cytotoxicity of EnP was evaluated.
The antimicrobial property was tested on (ATCC 35668), (ATCC 9222), (ATCC 35037), and (ATCC 29212), using the microbroth dilution method. The 0.2% Chlorhexidin (CHX) mouthwash was used as the control group. Besides, the cytotoxicity analysis was done on gingival fibroblasts by the MTT colorimetric method. The data were reported using descriptive methods, and analyzed by one-way ANOVA, and Tukey's HSD test.
The strongest bacteriostatic and bactericidal effects of and were observed for and , respectively (with the MIC and MBC value being 62.5 μg/mL). The antibacterial properties of EnP were comparable to those of CHX, being several times stronger than medicinal plants (1-14 μg/mL). Based on the cytotoxicity evaluation, there was no statistically significant difference observed between the cytotoxicity of the control group and that of Enp for three evaluations, except after 72 hours when the cell viability at the concentration of 3.75 µg/ml was significantly lower than that of the control group (=0.05). However, no concentration of EnP was observed to be over 50% of the growth inhibition (IC50) of the fibroblasts for the three evaluations.
EnP could be utilized in dental materials as a natural and safe antimicrobial agent against oral streptococci and E. faecalis, being as effective as CHX mouthwash. Antimicrobial peptide, Bacteriocin Entrocin P, Chlorhexidine, Cuminum cyminum, Enterococcus faecalis, Ferulago angulata.
本研究旨在评估细菌素肠球菌素P（EnP）重组肽、孜然精油和阿魏提取物对某些口腔病原体的抗菌性能。此外，还评估了EnP的细胞毒性。
采用微量肉汤稀释法对变形链球菌（ATCC 35668）、金黄色葡萄球菌（ATCC 9222）、粪肠球菌（ATCC 35037）和白色念珠菌（ATCC 29212）进行抗菌性能测试。使用0.2%氯己定（CHX）漱口水作为对照组。此外，采用MTT比色法对牙龈成纤维细胞进行细胞毒性分析。数据采用描述性方法报告，并用单因素方差分析和Tukey's HSD检验进行分析。
分别观察到EnP和孜然精油对变形链球菌和粪肠球菌具有最强的抑菌和杀菌作用（MIC和MBC值均为62.5μg/mL）。EnP的抗菌性能与CHX相当，比药用植物（1 - 14μg/mL）强几倍。根据细胞毒性评估，除72小时后3.75μg/ml浓度下的细胞活力显著低于对照组（P = 0.05）外，三次评估中对照组和EnP的细胞毒性之间未观察到统计学显著差异。然而，三次评估中均未观察到EnP的任何浓度超过成纤维细胞生长抑制（IC50）的50%。
EnP可作为一种天然安全的抗口腔链球菌和粪肠球菌的抗菌剂用于牙科材料，其效果与CHX漱口水相当。抗菌肽、细菌素肠球菌素P、氯己定、孜然、粪肠球菌、阿魏。
