Department of Biology, Faculty of Science, University of Tabuk, Tabuk, Saudi Arabia.
IET Nanobiotechnol. 2022 Jun;16(4):145-157. doi: 10.1049/nbt2.12082. Epub 2022 Mar 30.
Marine seaweeds are known to have a potential role against microbial and pesticidal activities. Ulva lactuca, a green macroalgae extract analysed through gas chromatography mass spectrometry reveals 31 compounds. Resistance of mosquito vectors to synthetic insecticides remains a major problem. Discovering and applying natural agents to act against disease vectors is challenging. The activities of the extract and nano-fabricated green synthesised silver nanoparticles were checked for use against Aedes aegypti and Culex pipiens. The crude extract and synthesised silver nanoparticles exhibited a notable larvicidal effect, and very effective inhibition of pupal and adult emergence. Inhibition of adult emergence of Ae.aegypti was 97.7% and in Cu.pipiens, it was 93.3%. Our genotypic study of Deoxyribonucleic acid from treated larvae utilising random primers MA-09, MA-12 and MA-26 revealed damaged nucleotide sequences when compared with the controls. The antimicrobial activity of both the extract and green synthesised nanomaterials showed prominent activity against pathogenic drug resistant bacteria. Our results contribute to further development of eco-friendly insecticides with lower cost of preparation. This could further contribute to further research helping future generations to be free from these deadly disease-causing vectors and pathogenic microbes.
海洋藻类被认为具有潜在的抗菌和抗农药活性。通过气相色谱-质谱分析，发现浒苔提取物中有 31 种化合物。蚊子对合成杀虫剂的抗药性仍然是一个主要问题。发现并应用天然药物来对抗病媒是具有挑战性的。本研究检测了提取物和纳米制造的绿色合成银纳米颗粒对埃及伊蚊和致倦库蚊的活性。粗提物和合成的银纳米颗粒对幼虫具有显著的杀蚊效果，对蛹和成虫的抑制效果非常显著。埃及伊蚊的成虫抑制率为 97.7%，库蚊为 93.3%。利用随机引物 MA-09、MA-12 和 MA-26 对处理过的幼虫进行 DNA 基因分型研究表明，与对照组相比，核苷酸序列受损。提取物和绿色合成纳米材料的抗菌活性均对具有抗药性的致病性细菌表现出显著的活性。我们的研究结果有助于进一步开发成本更低的环保型杀虫剂。这将有助于进一步的研究，使后代免受这些致命的病媒和致病微生物的侵害。
