Choi Jeong Su, Jung Hyon Chel, Baek Yeon Jae, Kim Bo Yong, Lee Min Woo, Kim Hyeong Dong, Kim Suhng Wook
School of Health and Environmental Science, College of Health Science, Korea University, Seoul 02841, Korea.
BK21 FOUR R&E Center for Learning Health Systems, Korea University, Seoul 02841, Korea.
Nanomaterials (Basel). 2021 Jan 14;11(1):205. doi: 10.3390/nano11010205.
In this work, the antibacterial activity of silver nanoparticles (AgNPs) synthesized using extracts against three species of antibiotic-susceptible and three species of resistant bacteria was investigated. The effects of this plant were more promising when compared with other medicinal plants tested. The hydrothermal extract of was mixed with silver nitrate to synthesize AgNPs. The synthesized particle characteristics were analyzed by UV-Vis spectrophotometry, scanning electron microscopy (SEM), dynamic light scattering (DLS), and Fourier-transform infrared spectroscopy (FT-IR). Minimum inhibitory concentration and minimum bactericidal concentration tests were conducted to confirm antibacterial activity and the results showed that AgNPs synthesized using extracts effectively inhibited the growth of bacterial species. Moreover, the SEM images of the bacterial species treated with AgNPs synthesized with extracts showed that clusters of AgNPs were attached to the surface of the bacterial cell wall, which could induce destruction of the cell membranes. The results suggest that AgNPs synthesized with extracts have the potential to treat antibiotic-resistant bacteria known as the major cause of nosocomial infections.
在本研究中，对使用提取物合成的银纳米颗粒（AgNPs）针对三种抗生素敏感细菌和三种耐药细菌的抗菌活性进行了研究。与其他测试的药用植物相比，这种植物的效果更具前景。将的水热提取物与硝酸银混合以合成AgNPs。通过紫外可见分光光度法、扫描电子显微镜（SEM）、动态光散射（DLS）和傅里叶变换红外光谱（FT-IR）对合成颗粒的特性进行了分析。进行了最低抑菌浓度和最低杀菌浓度测试以确认抗菌活性，结果表明使用提取物合成的AgNPs有效抑制了细菌种类的生长。此外，用提取物合成的AgNPs处理的细菌种类的SEM图像显示，AgNPs簇附着在细菌细胞壁表面，这可能导致细胞膜的破坏。结果表明，用提取物合成的AgNPs有潜力治疗作为医院感染主要原因的耐药细菌。
