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通过机械变形形成细胞封闭端隧道纳米管。

Formation of cellular close-ended tunneling nanotubes through mechanical deformation.

作者信息

Chang Minhyeok, Lee O-Chul, Bu Gayun, Oh Jaeho, Yunn Na-Oh, Ryu Sung Ho, Kwon Hyung-Bae, Kolomeisky Anatoly B, Shim Sang-Hee, Doh Junsang, Jeon Jae-Hyung, Lee Jong-Bong

机构信息

Department of Physics, Pohang University of Science and Technology (POSTECH), Pohang 37673, Korea.

POSTECH Biotech Center, Pohang 37673, Korea.

出版信息

Sci Adv. 2022 Apr;8(13):eabj3995. doi: 10.1126/sciadv.abj3995. Epub 2022 Mar 30.

DOI:10.1126/sciadv.abj3995
PMID:35353579
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8967236/
Abstract

Membrane nanotubes or tunneling nanotubes (TNTs) that connect cells have been recognized as a previously unidentified pathway for intercellular transport between distant cells. However, it is unknown how this delicate structure, which extends over tens of micrometers and remains robust for hours, is formed. Here, we found that a TNT develops from a double filopodial bridge (DFB) created by the physical contact of two filopodia through helical deformation of the DFB. The transition of a DFB to a close-ended TNT is most likely triggered by disruption of the adhesion of two filopodia by mechanical energy accumulated in a twisted DFB when one of the DFB ends is firmly attached through intercellular cadherin-cadherin interactions. These studies pinpoint the mechanistic questions about TNTs and elucidate a formation mechanism.

摘要

连接细胞的膜纳米管或隧道纳米管（TNTs）已被公认为是远距离细胞间运输的一种先前未被识别的途径。然而，这种延伸数十微米且能保持数小时稳定的精细结构是如何形成的尚不清楚。在这里，我们发现TNT是由两个丝状伪足通过物理接触形成的双丝状伪足桥（DFB）经螺旋变形发展而来的。当DFB的一端通过细胞间钙黏蛋白 - 钙黏蛋白相互作用牢固附着时，扭曲的DFB中积累的机械能破坏了两个丝状伪足之间的黏附，最有可能触发DFB向封闭端TNT的转变。这些研究明确了关于TNTs的机制问题，并阐明了一种形成机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c70/8967236/058ebde84d1b/sciadv.abj3995-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c70/8967236/db8b06ff67a1/sciadv.abj3995-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c70/8967236/09a1755880af/sciadv.abj3995-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c70/8967236/e4e458a3c12e/sciadv.abj3995-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c70/8967236/eafaa5814bb2/sciadv.abj3995-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c70/8967236/44f493f7df4e/sciadv.abj3995-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c70/8967236/058ebde84d1b/sciadv.abj3995-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c70/8967236/db8b06ff67a1/sciadv.abj3995-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c70/8967236/09a1755880af/sciadv.abj3995-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c70/8967236/e4e458a3c12e/sciadv.abj3995-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c70/8967236/eafaa5814bb2/sciadv.abj3995-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c70/8967236/44f493f7df4e/sciadv.abj3995-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c70/8967236/058ebde84d1b/sciadv.abj3995-f6.jpg

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