Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
Huoqiu Animal Health Supervision Institute, Animal Husbandry Development Center, Auditorium Road, Huoqiu County, Luan, Anhui 237400, China.
J Proteomics. 2022 May 30;260:104574. doi: 10.1016/j.jprot.2022.104574. Epub 2022 Mar 27.
Protein phosphorylation plays an important role in animal reproduction. However, its role in the onset of puberty in goats remains largely unexplored. Accordingly, in the present study, the molecular changes controlling the onset of puberty in goats were investigated by identifying the differentially phosphorylated proteins (DPPs) and phosphorylation sites (DPSs) in the hypothalami of prepubertal and pubertal female goats using LC-MS/MS and tandem mass tag labelling. A total of 3265 phosphopeptides corresponding to 1628 phosphoproteins were identified, including 234 upregulated and 342 downregulated phosphopeptides. The DPSs HTT, MAP1B, CAMKK1, MAP2, DNAJC5, and GAP43 were identified. These DPPs are enriched in the endocytosis, cAMP signaling, Rap1 signaling, melanogenesis, and insulin secretion pathways. These pathways are related to gonadotropin-releasing hormone and puberty. In particular, glucose-6-phosphate isomerase, fructose-bisphosphate aldolase C, and fructose-bisphosphate aldolase A occupy important locations in the protein-protein interaction network. These data provide evidence for a complex interaction network in goat hypothalamus proteins that affects puberty. Furthermore, they may help identify new puberty-regulating candidates and/or serve as an important resource for exploring the physiological mechanism of puberty onset in mammals. SIGNIFICANCE: This study provides evidence for a complex interaction network in goat hypothalamus proteins that affects puberty. Furthermore, our data may help identify new puberty-regulating candidates and/or serve as an important resource for exploring the physiological mechanism of puberty onset in mammals.
蛋白质磷酸化在动物繁殖中起着重要作用。然而，其在山羊青春期开始中的作用在很大程度上仍未得到探索。因此，在本研究中，通过使用 LC-MS/MS 和串联质量标签标记，鉴定了青春期前和青春期雌性山羊下丘脑控制青春期开始的分子变化，从而研究了调控山羊青春期开始的分子变化。共鉴定到 3265 个对应于 1628 个磷酸化蛋白的磷酸肽，其中 234 个上调和 342 个下调磷酸肽。鉴定到了 DPSs HTT、MAP1B、CAMKK1、MAP2、DNAJC5 和 GAP43。这些 DPP 富集在胞吞作用、cAMP 信号转导、Rap1 信号转导、黑色素生成和胰岛素分泌途径中。这些途径与促性腺激素释放激素和青春期有关。特别是葡萄糖-6-磷酸异构酶、果糖二磷酸醛缩酶 C 和果糖二磷酸醛缩酶 A 在蛋白质-蛋白质相互作用网络中占据重要位置。这些数据为影响青春期的山羊下丘脑蛋白复杂相互作用网络提供了证据。此外，它们可能有助于鉴定新的青春期调节候选物，或作为探索哺乳动物青春期开始生理机制的重要资源。意义：本研究为影响青春期的山羊下丘脑蛋白复杂相互作用网络提供了证据。此外，我们的数据可能有助于鉴定新的青春期调节候选物，或作为探索哺乳动物青春期开始生理机制的重要资源。
