定量蛋白质组学分析评估青春期山羊卵巢中的蛋白质表达水平。
Quantitative proteomics analysis to assess protein expression levels in the ovaries of pubescent goats.
机构信息
Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, Anhui, China.
Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, Anhui, China.
出版信息
BMC Genomics. 2022 Jul 13;23(1):507. doi: 10.1186/s12864-022-08699-y.
BACKGROUND
Changes in the abundance of ovarian proteins play a key role in the regulation of reproduction. However, to date, no studies have investigated such changes in pubescent goats. Herein we applied isobaric tags for relative and absolute quantitation (iTRAQ) and liquid chromatography-tandem mass spectrometry to analyze the expression levels of ovarian proteins in pre-pubertal (n = 3) and pubertal (n = 3) goats.
RESULTS
Overall, 7,550 proteins were recognized; 301 (176 up- and 125 downregulated) were identified as differentially abundant proteins (DAPs). Five DAPs were randomly selected for expression level validation by Western blotting; the results of Western blotting and iTRAQ analysis were consistent. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that DAPs were enriched in olfactory transduction, glutathione metabolism, and calcium signaling pathways. Besides, gene ontology functional enrichment analysis revealed that several DAPs enriched in biological processes were associated with cellular process, biological regulation, metabolic process, and response to stimulus. Protein-protein interaction network showed that proteins interacting with CDK1, HSPA1A, and UCK2 were the most abundant.
CONCLUSIONS
We identified 301 DAPs, which were enriched in olfactory transduction, glutathione metabolism, and calcium signaling pathways, suggesting the involvement of these processes in the onset of puberty. Further studies are warranted to more comprehensively explore the function of the identified DAPs and aforementioned signaling pathways to gain novel, deeper insights into the mechanisms underlying the onset of puberty.
背景
卵巢蛋白丰度的变化在生殖调控中起着关键作用。然而，迄今为止，尚未有研究调查过青春期前山羊卵巢蛋白的这种变化。在此，我们应用相对和绝对定量同位素标记（iTRAQ）和液相色谱-串联质谱技术分析了青春期前（n = 3）和青春期（n = 3）山羊卵巢蛋白的表达水平。
结果
共鉴定到 7550 种蛋白质；其中 301 种（176 种上调和 125 种下调）被鉴定为差异丰度蛋白（DAP）。随机选择 5 个 DAP 进行 Western blot 验证；Western blot 和 iTRAQ 分析结果一致。京都基因与基因组百科全书通路富集分析表明，DAP 富集于嗅觉转导、谷胱甘肽代谢和钙信号通路。此外，基因本体功能富集分析表明，一些 DAP 富集于与细胞过程、生物调节、代谢过程和对刺激的反应相关的生物学过程。蛋白质-蛋白质相互作用网络显示，与 CDK1、HSPA1A 和 UCK2 相互作用的蛋白质最为丰富。
结论
我们鉴定到 301 个 DAP，这些 DAP 富集于嗅觉转导、谷胱甘肽代谢和钙信号通路，提示这些过程参与了青春期的启动。进一步的研究需要更全面地探索所鉴定的 DAP 及其上述信号通路的功能，以深入了解青春期启动的机制。
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