Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland.
Sci Data. 2022 Mar 30;9(1):120. doi: 10.1038/s41597-022-01242-4.
We present a dataset of microelectrode array (MEA) recordings from human pluripotent stem cell (hPSC)-derived and rat embryonic cortical neurons during their in vitro maturation. The data were prepared to assess extracellularly recorded spontaneous activity and to compare the functional development of these neuronal networks. In addition to recordings of spontaneous activity, we provide pharmacological responses of hPSC-derived and rat cortical cultures at their mature stage. Together with the recorded electrode raw data, we share the analysis code to form a comprehensive dataset including spike times, spike waveforms, burst activity and network synchronization metrics calculated with two different connectivity estimators. Moreover, we provide the analysis code that produced the key scientific findings published previously with this dataset. This large dataset enables investigation of the functional aspects of maturing cortical neuronal networks and provides substantial parameters to assess the differences and similarities between hPSC-derived and rat cortical networks in vitro. This publicly available dataset will be beneficial, especially for experimental and computational neuroscientists.
我们提供了一组微电极阵列 (MEA) 记录，这些记录来自人类多能干细胞 (hPSC) 衍生的和大鼠胚胎皮质神经元在体外成熟过程中的记录。这些数据旨在评估细胞外记录的自发活动，并比较这些神经元网络的功能发育。除了自发活动的记录外，我们还提供了 hPSC 衍生和大鼠皮质培养物在成熟阶段的药理学反应。与记录的电极原始数据一起，我们分享了分析代码，形成了一个综合数据集，包括用两种不同的连接估计器计算的尖峰时间、尖峰波形、爆发活动和网络同步度量。此外，我们还提供了产生以前使用该数据集发表的关键科学发现的分析代码。这个大型数据集能够研究成熟皮质神经元网络的功能方面，并提供大量参数来评估 hPSC 衍生和大鼠皮质网络在体外的差异和相似之处。这个公开可用的数据集将特别有益于实验和计算神经科学家。
