Liu Yi-Rong, Sun Wei-Zheng, Wu Jian
School of Medicine, Tsinghua University, Beijing, China.
Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, China.
Front Bioeng Biotechnol. 2022 Mar 9;10:841298. doi: 10.3389/fbioe.2022.841298. eCollection 2022.
The clinical tissue bulks' surface is always coarse and shows a complex microscopic geometry which may affect the visual effect of polarization images and calculation of polarization parameters of the sample. To confirm whether this effect would cause identification difficulties and misjudgments on the target recognition when performed the polarization imaging based on 3 × 3 Mueller matrix measurement, cylindrical type and slope type physical models were used to study and analyze the effect of the surface with complex microscopic geometry on the polarization images. Then, clinical tumor bulk samples were used to interact with different sizes of patterns to simulate the different complex microscopic geometry and test the coarse surface effect on polarization images. Meanwhile, assessment parameters were defined to evaluate and confirm the variation between two polarization images quantitatively. The results showed that the polarization imaging of the sample surface with the complex microscopic geometry led to acceptable visual effect and limited quantitative variation on the value of polarization parameters and assessment parameters, and it caused no identification difficulties on target recognition, indicating that it is feasible to apply the polarization imaging based on 3 × 3 Mueller matrix measurement on clinical tissues with the complex microscopic geometry sample surface.
临床组织块的表面通常较为粗糙，呈现出复杂的微观几何结构，这可能会影响偏振图像的视觉效果以及样品偏振参数的计算。为了确定在基于3×3穆勒矩阵测量进行偏振成像时，这种影响是否会导致目标识别出现困难和误判，我们使用圆柱形和倾斜型物理模型来研究和分析具有复杂微观几何结构的表面对偏振图像的影响。然后，使用临床肿瘤组织块样本与不同尺寸的图案相互作用，以模拟不同的复杂微观几何结构，并测试粗糙表面对偏振图像的影响。同时，定义评估参数以定量评估和确认两幅偏振图像之间的差异。结果表明，具有复杂微观几何结构的样品表面的偏振成像产生了可接受的视觉效果，偏振参数和评估参数值的定量变化有限，并且在目标识别上没有造成识别困难，这表明基于3×3穆勒矩阵测量的偏振成像应用于具有复杂微观几何结构样品表面的临床组织是可行的。
