Nazir Muhammad Mudassir, Li Qi, Noman Muhammad, Ulhassan Zaid, Ali Shafaqat, Ahmed Temoor, Zeng Fanrong, Zhang Guoping
Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
Institute of Biotechnology, Zhejiang University, Hangzhou, China.
Front Plant Sci. 2022 Mar 11;13:843795. doi: 10.3389/fpls.2022.843795. eCollection 2022.
Arsenic (As) contamination in agricultural soils has become a great threat to the sustainable development of agriculture and food safety. Although a lot of approaches have been proposed for dealing with soil As contamination, they are not practical in crop production due to high cost, time-taking, or operational complexity. The rapid development of nanotechnology appears to provide a novel solution to soil As contamination. This study investigated the roles of calcium oxide nanoparticles (CaO NPs) in alleviating As toxicity in two barley genotypes (LJZ and Pu-9) differing in As tolerance. The exposure of barley seedlings to As stress showed a significant reduction in plant growth, calcium and chlorophyll content (SPAD value), fluorescence efficiency (, and a dramatic increase in the contents of reactive oxygen species (ROS), malondialdehyde (MDA) and As, with LJZ being more affected than Pu-9. The exogenous supply of CaO NPs notably alleviated the toxic effect caused by As in the two barley genotypes. Moreover, the expression of As transporter genes, that is, , , and , was dramatically enhanced when barley seedlings were exposed to As stress and significantly reduced in the treatment of CaO NPs addition. It may be concluded that the roles of CaO NPs in alleviating As toxicity could be attributed to its enhancement of Ca uptake, ROS scavenging ability, and reduction of As uptake and transportation from roots to shoots.
农业土壤中的砷（As）污染已对农业可持续发展和食品安全构成重大威胁。尽管已提出许多处理土壤砷污染的方法，但由于成本高、耗时或操作复杂，它们在作物生产中并不实用。纳米技术的快速发展似乎为土壤砷污染提供了一种新的解决方案。本研究调查了氧化钙纳米颗粒（CaO NPs）在两种耐砷性不同的大麦基因型（LJZ和Pu-9）中缓解砷毒性的作用。大麦幼苗暴露于砷胁迫下，植株生长、钙和叶绿素含量（SPAD值）、荧光效率显著降低，活性氧（ROS）、丙二醛（MDA）和砷含量显著增加，LJZ比Pu-9受影响更大。外源供应CaO NPs显著缓解了两种大麦基因型中砷引起的毒性效应。此外，当大麦幼苗暴露于砷胁迫时，砷转运基因（即 、 、 和 ）的表达显著增强，而在添加CaO NPs的处理中显著降低。可以得出结论，CaO NPs缓解砷毒性的作用可能归因于其增强了钙的吸收、ROS清除能力以及减少了砷从根到地上部的吸收和运输。
