State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China.
J Am Chem Soc. 2013 Feb 27;135(8):2903-6. doi: 10.1021/ja311688g. Epub 2013 Feb 19.
We have developed Lyso-V, the first fluorescent probe of lysosomal viscosity. Because of its lysosome-actived fluorescence characteristics, Lyso-V has proved to be an ideal lysosomal tracer with high spatial and temporal resolution under laser confocal microscopy. More importantly, Lyso-V shows its practical applicability in real-time quantification of lysosomal viscosity changes in live cells through fluorescence lifetime imaging microscopy.
我们开发了 Lyso-V，这是第一个溶酶体粘度的荧光探针。由于其具有溶酶体激活荧光特性，因此 Lyso-V 在激光共聚焦显微镜下已被证明是一种具有高时空分辨率的理想溶酶体示踪剂。更重要的是，Lyso-V 通过荧光寿命成像显微镜显示了其在实时定量活细胞溶酶体粘度变化方面的实际应用。
