Vascular Biology Program, Boston Children's Hospital, Boston, Massachusetts, USA.
Department of Surgery, Harvard Medical School, Boston, Massachusetts, USA.
J Extracell Vesicles. 2022 Apr;11(4):e12202. doi: 10.1002/jev2.12202.
With an exponential increase in extracellular vesicle (EV) studies in the past decade, focus has been placed on standardization of experimental design to ensure inter-study comparisons and validity of conclusions. In the case of in vitro assays, the composition of cell culture media is important to consider for EV studies. In particular, levels of lipoproteins, which are critical components of the interstitial fluid, should be taken into consideration. Results from this study reveal that lipoprotein levels in cell culture medium impact the effects that EVs have on recipient cells. Additionally, evidence of EV binding and fusion to lipoprotein-like structures in plasma is provided. However, it is unclear whether the impact of lipoproteins in cell culture is due to direct interactions with EVs, indirect effects, or a combination of both mechanisms. Taken together, cell culture studies performed in the absence of physiological levels of lipoproteins are unlikely to reflect interactions that occur between EVs and recipient cells in an in vivo environment.
在过去十年中，细胞外囊泡 (EV) 的研究呈指数级增长，人们关注的重点是标准化实验设计，以确保研究间的比较和结论的有效性。在体外分析中，细胞培养培养基的组成对于 EV 研究很重要。特别是，应该考虑到作为细胞间质重要组成部分的脂蛋白的水平。本研究结果表明，细胞培养培养基中的脂蛋白水平会影响 EV 对受体细胞的作用。此外，还提供了 EV 与血浆中类似脂蛋白结构结合和融合的证据。然而，尚不清楚细胞培养中脂蛋白的影响是由于与 EV 的直接相互作用、间接影响还是这两种机制的结合。总之，在缺乏生理水平脂蛋白的情况下进行的细胞培养研究不太可能反映 EV 与体内环境中受体细胞之间发生的相互作用。
