Xu Wenjing, Xuan Guanhua, Lin Hong, Wang Jingxue
Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China.
Arch Virol. 2022 May;167(5):1311-1316. doi: 10.1007/s00705-022-05413-x. Epub 2022 Apr 1.
Here, we describe the characterization and genome annotation of the newly isolated lytic Vibrio parahaemolyticus phage vB_VpP_WS1, isolated from sewage samples collected in Qingdao, China. Transmission electron microscopy revealed that vB_VpP_WS1 is about 22 nm in size and that the virions are isometric, likely icosahedral, particles similar to those of members of the Microviridae. The digestion patterns of phage nucleic acids and whole-genome sequencing analysis together revealed that phage vB_VpP_WS1 has a single-stranded DNA genome of 5564 nt. Eight open reading frames were identified, only four of which could be annotated. The proteins of vB_VpP_WS1 displayed low sequence similarity to their homologs encoded by other microviruses. Phylogenetic analysis based on the major capsid protein suggested that vB_VpP_WS1 is a tentative new member of the family Microviridae.
在此，我们描述了新分离的溶菌性副溶血性弧菌噬菌体vB_VpP_WS1的特征及基因组注释，该噬菌体是从中国青岛采集的污水样本中分离得到的。透射电子显微镜显示，vB_VpP_WS1大小约为22纳米，病毒粒子呈等轴状，可能为二十面体，类似于微小病毒科成员的粒子。噬菌体核酸的消化模式和全基因组测序分析共同表明，噬菌体vB_VpP_WS1具有一个5564个核苷酸的单链DNA基因组。鉴定出八个开放阅读框，其中只有四个能够进行注释。vB_VpP_WS1的蛋白质与其由其他微小病毒编码的同源物显示出低序列相似性。基于主要衣壳蛋白的系统发育分析表明，vB_VpP_WS1是微小病毒科的一个暂定新成员。
