Yoo Kwon Jun, Jang Woo Young, Chang Jeong Ho
Korea Institute of Ceramic Engineering and Technology, Jinju 52851, Republic of Korea.
ACS Omega. 2024 Oct 17;9(43):43678-43688. doi: 10.1021/acsomega.4c06020. eCollection 2024 Oct 29.
In this study, S53P4 (53SiO-23NaO-20CaO-4PO) bioactive glass (BG) were prepared through a melt-milling process, and their bioresorption and biomineralization behavior was evaluated by in vitro dissolution under different solution conditions (neutral and acidic). The particle size of S53P4 BG was controlled by milling, and the in vitro dissolution evaluation was performed in tris buffer and citric acid solution for 21 days at 37 °C according to ISO 10993-14 (biological evaluation of medical devices). During dissolution, the ion release rate of S53P4 BG was confirmed to be three times faster in citric acid solution than that in tris buffer. Among them, the ion concentration of calcium and phosphorus initially increased and then gradually decreased, which is due to the biomineralization process. This process formed a new layer of particles on the surface of S53P4 BG, which was identified as a calcium-phosphate-based compound by X-ray diffraction analysis. Furthermore, the thickness of the layer was observed to be 273 nm in tris buffer and 34 nm in citric acid solution by focused-ion beam scanning electron microscopy, and the morphology of the particles comprising this layer was observed to be thicker and longer in tris buffer than that in citric acid solution. This difference is due to the citrate present in the citric acid solution interacting with the released calcium ions and inhibiting the formation of a new layer. Thus, the ion release of S53P4 BG was faster in citric acid solution than that in tris buffer, but the biomineralization process to form the calcium phosphate-based compound was more effective in tris buffer.
在本研究中，通过熔融球磨工艺制备了S53P4（53SiO-23NaO-20CaO-4PO）生物活性玻璃（BG），并通过在不同溶液条件（中性和酸性）下的体外溶解来评估其生物吸收和生物矿化行为。通过球磨控制S53P4 BG的粒径，并根据ISO 10993-14（医疗器械生物学评价）在37℃下于三羟甲基氨基甲烷缓冲液和柠檬酸溶液中进行21天的体外溶解评估。在溶解过程中，证实S53P4 BG在柠檬酸溶液中的离子释放速率比在三羟甲基氨基甲烷缓冲液中快三倍。其中，钙和磷的离子浓度最初增加，然后逐渐降低，这是由于生物矿化过程所致。该过程在S53P4 BG表面形成了一层新的颗粒，通过X射线衍射分析鉴定为磷酸钙基化合物。此外，通过聚焦离子束扫描电子显微镜观察到，在三羟甲基氨基甲烷缓冲液中该层的厚度为273 nm，在柠檬酸溶液中为34 nm，并且观察到构成该层的颗粒形态在三羟甲基氨基甲烷缓冲液中比在柠檬酸溶液中更厚更长。这种差异是由于柠檬酸溶液中存在的柠檬酸盐与释放的钙离子相互作用并抑制了新层的形成。因此，S53P4 BG在柠檬酸溶液中的离子释放比在三羟甲基氨基甲烷缓冲液中更快，但在三羟甲基氨基甲烷缓冲液中形成磷酸钙基化合物的生物矿化过程更有效。
